Mechanism for the induction of 3-hydroxy-3-methylglutaryl coenzyme A reductase in HgCl_2-treated sweet potaso root tissue

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No 3-hydroxy-3-methylglutaryl coenzyme A reductase activity was detected in microsomal fractions prepared from healthy and wounded sweet potato root tissues. However, there was considerable enzyme activity in the tissue discs when HgCl_2 was applied after incubation at 30℃ for 18 hr. This increase in the enzyme activity was followed by furano-terpene accumulation. Application of cycloheximide to discs immediately after preparation completely inhibited the increase in the enzyme activity when HgCl_2 was applied after incubation. In contrast, the increase was delayed for about 4 hr, then the activity was enhanced, when CHI was applied after preliminary incubation. CHI completely inhibited protein synthesis when applied to the discs after the preliminary incubation, as judged by the inhibition of the incorporation of ^<14>C-leucine into protein and the inhibition of the increase in peroxidase activity which is synthesized de novo. These results suggest that the inactive precursor of HMG-CoA reductase is synthesized during the preliminary incubation in response only to wounding then it is converted into the active form after treatment with HgCl_2.
日本植物生理学会の論文