Glucose 6-phosphate dehydrogenase from sweet potato : Effect of various ions and their ionic strength on enzyme activity
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概要
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Activity of glucose 6-phosphate dehydrogenase (D-glucose 6-phosphate : NADP oxidoreductase, EC 1.1.1.49) preparation from sweet potato root tissue was markedly altered in the presence of various ions. Cations or anions were effective in the following order : Na^+, K^+ > Tris^+ > NH_4^+ > Mg^<2+> > Ca^<2+>, or Cl^- >NO_3^-, HPO_4^<2-> > SO_4^<2-> > HCO_3^-. Activity was inhibited at high concentrations of Ca^<2+> and HCO_3^-. In an investigation on the dependence of the activity on pH, two activity peaks were clearly observed at low ionic strength. Ionic strength altered both the Km and Vmax for glucose 6-phosphate (G6P). A Lineweaver-Burk plot for the enzyme, with respect to G6P, showed a bimodal nature at low ionic strength; suggesting negative cooperativity. Deviation from linearity of the plot was less with an increase in the ionlc strength.
- 日本植物生理学会の論文
著者
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Uritani Ikuzo
Laboratory Of Biochemistry And Institute For Biochemical Regulation Faculty Of Agriculture Nagoya Un
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Muto Shoshi
Laboratory Of Biochemistry Faculty Of Agriculture Nagoya University:(present)institute Of Applied Mi
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Muto Shoshi
Laboratory Of Biochemistry Faculty Of Agriculture Nagoya University
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