Mechanism of the Increase in Succinate Dehydrogenase Activity in Wounded Sweet Potato Root Tissue
スポンサーリンク
概要
- 論文の詳細を見る
The large subunit (mol wt: 65,000) of sweet potato succinate dehydrogenase was isolated by SDS-polyacrylamide gel electrophoresis of a succinate dehydrogenase preparation, which had been partially purified from root mitochondria by solubilizing the enzyme with Emulgen 810, DEAE-cellulose column chromatography, and polyacrylamide gel electrophoresis. Antibody to the purified large subunit was produced in a rabbit, and the antiserum obtained was judged to be specific to the large subunit based on the results of double immunodiffusion tests and immunoelectrophoresis. Rocket immunoelectrophoresis with the antiserum showed that the increase in succinate dehydrogenase activity during the ageing of sliced, sweet potato root tissue was due to an increase in the amount of enzyme protein. Both the increases in the activity of succinate dehydrogenase and in the amount of the large subunit protein were inhibited by cycloheximide or chloramphenicol. We propose that synthesis of the large subunit of succinate dehydrogenase on cytoplasmic ribosomes is controlled by a mitochondrial translation product(s).
- 日本植物生理学会の論文
著者
-
Hattori Tsukaho
Laboratory Of Biochemistry Faculty Of Agriculture Nagoya University
-
Asahi Tadashi
Laboratory Of Biochemistry Faculty Of Agriculture Nagoya University
-
Asahi Tadashi
Laboralory Of Biochemistry Faculty Of Agriculture Nagoya University
関連論文
- Evidence for No Proteolytic Processing during Transport of Isocitrate Lyase into Glyoxysomes in Castor Bean Endosperm
- A Family of Potato Genes That Encode Kunitz-Type Proteinase Inhibitors : Structural Comparisons and Differential Expression : GENES STRUCTURE AND EXPRESSION
- Subcellular Localization of the Sweet Potato Tuberous Root Storage Protein(Biological Chemistry)
- Effect of the pea embryo on formation and degeneration of the mitochondrial membrane in cotyledons during germination
- Sucrose-Induced Expression of Genes Coding for the Tuberous Root Storage Protein, Sporamin, of Sweet Potato in Leaves and Petioles
- Tissue-Specific Isoforms of Catalase Subunits in Castor Bean Seedlings
- Different Sets of cis-Elements Contribute to the Expression of a Catalase Gene from Castor Bean during Seed Formation and Postembryonic Development in Transgenic Tobacco : GENE STRUCTURE AND EXPRESSION
- Differential Regulation of the Expression in Transgenic Tobacco of the Gene for β-Glucuronidase under the Control of the 5'-Upstream Regions of Two Catalase Genes from Castor Bean : GENE STRUCTURE AND EXPRESSION
- Construction and Expression in Tobacco of a β-Glucuronidase (GUS) Reporter Gene Containing an Intron Within the Coding Sequence
- Isolation and Properties of Intact Chromoplasts from Tomato Fruits
- Properties of the Dicyclohexylcarbodiimide-Binding Subunit of Cytochrome c Oxidase from Sweet Potato
- N-Terminal Amino Acid Sequence and Processing Site of Sweet Potato Cytochrome c Oxidase Subunit II
- Differences in the Thermostability and Subunit Species of Cytochrome c Oxidase among Tissues
- Purification and Properties of Sweet Potato NADPH-Cytochrome c (P-450) Reductase
- Different Intracellular Localization of Two Cytochrome P-450 Systems, Ipomeamarone 15-Hydroxylase and Cinnamic Acid 4-Hydroxylase, in Sweet Potato Root Tissue Infected with Ceratocystis fimbriata
- Suppression by Exogenous Phospholipid of Cyanide-Insensitive Respiration of Submitochondrial Particles from Sweet Potato Root Tissue
- Immunological Studies on ATPases in Mung Bean Hypocotyl Plasma Membrane : Proposal of the Presence of Two Molecular Species of ATPase
- Nucleotide Sequence of cDNA for Concanvalin A from Canavalia gladiata Seeds
- The Presequence of a Precursor to the (δ-Subunit of Sweet Poteto Mitochondrial F_1 ATPase is not Sufficient for the Transport of β-Glucuronidase (GUS) into Mitochondria of Tobacco, Rice and Yeast Cells
- A Comparison of Tomato Fruit Chloroplast and Chromoplast DNAs as Analyzed with Restriction Endonucleases
- Partial Purification and Characterization of An ATPase in Mung Bean Hypocotyl Plasma Membrane : Suggestion for A New Type of Higher Plant Plasma Membrane ATPase
- CHANGE OF B-TYPE HAEM CONTENT IN RELATION TO PEROXIDASE BIOSYNTHESIS IN INJURED SWEET POTATO ROOTS
- Heterogeneity of mitochondrial particles in fresh and wounded tissues of sweet potato roots
- Increase in Catalase mRNA in Wounded Sweet Potato Tuberous Root Tissue
- The Presence of Two Forms of Succinate Dehydrogenase in Sweet Potato Root Mitochondria
- Biochemical studies on biogenesis of mitochondria in wounded sweet potato root tissue I. Time course analysis of increase in mitochondrial enzymes
- A Complete Rat Serum Albumin cDNA Clone Directly Identified by Immunological Screening of cDNA Expression Library(Biological Chemistry)
- Degenerative changes in properties of the mitochondrial inner membrane in pea cotyledons during germination
- Modification of OGUR-ROSEN method for estimation of RNA in fresh and wounded sweet potato root tissues and change in RNA contents after the cutting
- Effect of antibiotics on biogenesis of mitochondria during aging of sliced sweet potato root tissue
- Effect of Ethylene on the Increase in Catalase Activity through Microbody Development in Wounded Sweet Potato Root Tissue
- Isolation of microbodies from sweet potato root tissue and their development during aging of slices
- Mechanism of the Increase in Catalase Activity through Microbody Development in Wounded Sweet Potato Root Tissue
- Changes in mitochondrial activity during enlargement of mung bean root cells
- Increase in mitochondrial activity in diseased sweet potato root tissue
- Membranes carrying acid hydrolases in pea seedling roots
- Activities of RNases in different cell compartments in different regions of pea roots
- Biochemical studies on biogenesis of mitochondria in wounded sweet potato root tissue II. Active synthesis of membrane-bound protein of mitochondria
- Changes in Properties of Succinate Dehydrogenase during Senescence of Pea Cotyledons
- Mechanism of the Increase in Succinate Dehydrogenase Activity in Wounded Sweet Potato Root Tissue
- Isolation of membranes containing protease from the cotyledons of germinating pea seeds
- Purification and Properties of Catalase from Sweet Potato Root Microbodies
- Studies on Microbody Development in Wounded Sweet Potato Root Tissue : Proposal for the Post-Translational Transport of Catalase into Preexisting Microbodies
- An enzyme in mung bean leaves that damages cell organelles which leads to loss of their matrix enzymes
- Relationship between the water content of pea cotyledons and mitochondrial development during the early stage of germination
- Subcellular distribution of hydrolase in germinating pea cotyledons
- Biochemical studies on mitochondria formed during aging of sliced potato tuber tissue
- Non-synchronous increases in activities of peroxisomal enzymes in etiolated mung bean seedling leaves after illumination
- The Mode of Action of Organelle-Damaging Factor from Mung Bean Leaves
- Increases in activities of membrane-bound hydrolases in pea cotyledons during germination
- Effect of Phospholipid on Pea Cytochrome c Oxidase
- The Subunit Composition of Sweet Potato Cytochrome b-c1 Complex
- Induction of Polysome Formation in Sweet Potato Root Tissue in Response to Wounding