Different Intracellular Localization of Two Cytochrome P-450 Systems, Ipomeamarone 15-Hydroxylase and Cinnamic Acid 4-Hydroxylase, in Sweet Potato Root Tissue Infected with Ceratocystis fimbriata
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概要
- 論文の詳細を見る
Ipomeamarone 15-hydroxylase activity was mainly recovered in the pellet fraction between centrifugations at 10,000 and 100,000 ×g from a crude extract of Ceratocystis fimbriata-infected sweet potato root tissue, whereas cinnamic acid 4-hydroxylase activity was found between centrifugations at 300 and 10,000 ×g. When particles in the crude extract were fractionated by sucrose density gradient centrifugation, the rough-surfaced microsomes were distributed over a wide density range from 1.09 to 1.14g cm^<-3> judging from the distributions of protein, RNA and NADPH-cytochrome c reductase activity. Phosphorylcholine-glyceride transferase activity was only in the lighter half of the microsomal fraction (density: 1.09-1.11 g cm^<-3>). Ipomeamarone 15-hydroxylase activity was found in heavier half of the microsomal fraction (density: 1.10-1.14 g cm^<-3>). We propose that this tissue has two rough-surfaced endoplasmic reticulum species, only one of which carries phosphorylcholine-glyceride transferase, and that the cytochrome P-450 system is localized on the species lacking the enzyme. Cinnamic acid 4-hydroxylase activity was mainly found in a fraction that had densities of 1.17-1.19 g cm^<-3> and contained vesicular particles of various sizes.
- 日本植物生理学会の論文
著者
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Asahi Tadashi
Laboratory of Biochemistry, School of Agriculture, Nagoya University
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Fujita Masayuki
Laboratory of Food Hygienics, Faculty of Agriculture, Kagawa University
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Asahi T
Fukui Prefectural Univ. Fukui Jpn
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Asahi Tadashi
Laboratory Of Biochemistry Faculty Of Agriculture Nagoya University
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Fujita Masayuki
Laboratory Of Biochemistry Faculty Of Agriculture Nagoya University:(present)laboratory Of Food Hygi
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Fujita Masayuki
Laboratory Of Biochemistry Faculty Of Agriculture Nagoya University:(present)laboratory Of Food Hygi
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Asahi Tadashi
Laboralory Of Biochemistry Faculty Of Agriculture Nagoya University
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