The Presequence of a Precursor to the (δ-Subunit of Sweet Poteto Mitochondrial F_1 ATPase is not Sufficient for the Transport of β-Glucuronidase (GUS) into Mitochondria of Tobacco, Rice and Yeast Cells
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概要
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A precursor to the 6-subunit of sweet potato mitochondrial F_1ATPase (pre-F_1δ) has an amino-terminal (N-terminal) presequence of 45 amino acid residues and its N-terminal 18 residues may form an amphiphilic α-helix, which is typical of mitochondrial targeting signals [Kimura et al. (1990) J. Biol. Chem. 265: 6079]. Fusion genes consisting of sequences that encoded the 25 (DG25), 46 (DG46) and 73 (DG73) N-terminal amino acids from pre-F_1δ fused to the N-terminus of the coding sequence of bacterial β-glucuronidase (GUS) were placed downstream of the 35S promoter of cauliflower mosaic virus and used to transform suspension-cultured tobacco cells, rice calli and tobacco plants. Fusion genes were also placed downstream of the yeast GAL10 promoter and introduced into Saccharomyces cerevisiae cells. In these transformed cells, only the DG73 GUS-fusion protein was transported into mitochondria and subjected to proteolytic cleavage of the presequence. Neither transport to mitochondria nor processing of the presequence of the DG46 GUS-fusion protein, which contained the entire presequence and the processing site, occurred in either plant or yeast cells. These results indicate that the presequence of pre-F_1δ is not sufficient for the transport of the GUS protein into mitochondria in tobacco, rice and yeast cells. The requirement for the longer polypeptide from pre-F_1δ in the transport of the GUS protein into mitochondria could be due either to the lack of sufficient information for mitochondrial targeting within the presequence or to the nature of the passenger protein, GUS, used in this study.
- 日本植物生理学会の論文
著者
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NAKAMURA Kenzo
Laboratory of Biochemistry, Graduate School of Bioagricultural Sciences, Nagoya University
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Asahi Tadashi
Laboratory of Biochemistry, School of Agriculture, Nagoya University
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Takeda Shin
Laboratory of Biochemistry, School of Agriculture, Nagoya University
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SHIMAMOTO Ko
Laboratory of Plant Molecular Genetics, Nara Institute of Science and Technology
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SHIMAMOTO Ko
Lab.Plant Mol.Genet.NAIST
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Kimura Tetsuya
Laboratory of Applied Microbiology, Faculty of Bioresources, Mie University
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Kimura Tetsuya
Laboratory Of Biochemistry School Of Agriculture Nagoya University:(permanent)food Research Institut
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Shimamoto Ko
Plant Mol. Gen. Lab. Naist
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Asahi Tadashi
Laboratory Of Biochemistry Faculty Of Agriculture Nagoya University
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Asahi Tadashi
Laboratory Of Biochemistry School Of Agriculture Nagoya University:(present)faculty Of Biotechnology
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KYOZUKA Junko
Plantech Research Institute
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Kyozuka J
Graduate School Of Agricultural And Life Sciences The University Of Tokyo
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Kyozuka Junko
Plant Mol. Gen. Lab. Naist
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Shimamoto K
Laboratory Of Plant Molecular Genetics Nara Institute Of Science And Technology
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Nakamura Kenzo
Laboratory Of Biochemistry Faculty Of Agriculture Nagoya University
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Takeda Shin
Laboratory Of Biochemistry School Of Agriculture Nagoya University
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Asahi Tadashi
Laboralory Of Biochemistry Faculty Of Agriculture Nagoya University
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