Expression Patterns of Two Genes for the Delta-Subunit of Mitochondrial F_1-ATP Synthase from Sweet Potato in Transgenic Tobacco Plants and Cells

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Two nuclear genes, F_1δ-1 and F_1δ-2, coding for the δ-subunit of mitochondrial F_1-ATP synthase, which corresponds to oligomycin-sensitivity conferring protein in animal and yeast mitochondria, were isolated from sweet potato. The gene for the δ-subunit was composed of 6 exons and these two genes shared high sequence similarities to each other not only in exons but also in introns and in the 5'-upstream regions. However, the 5'-upstream regions of F_1δ-1 and F_1δ-2 were distinguishable by the presence of novel sequences, designated Ins-1 and Ins-2, respectively. Ins-1 and Ins-2 contained a terminal direct repeat of 1- bp and 12 bp, respectively, and various forms of repeat sequences. The promoter fusion of both F_1δ-1 and F_1δ-2 with the GUS coding sequence gave expression of GUS activity in transformed tobacco BY-2 cells, although the levels of GUS activity and the patterns of expression during the growth of cells were different between the two. In transgenic tobacco plants, the two fusion genes showed similar levels of expression in leaves and stems, while F_1δ-2:GUS gave significantly higher levels of expression in roots than F_1δ-1:GUS. Deletion of Ins-1 from the 5'-upstream region of F_1δ-1:GUS did not affect the expression of the fusion gene in various organs of transgenic plants. However, it caused significant enhancement of expression in transformed tobacco BY-2 cells.
日本植物生理学会の論文