Sucrose-Induced Expression of Genes Coding for the Tuberous Root Storage Protein, Sporamin, of Sweet Potato in Leaves and Petioles

スポンサーリンク

概要

• 論文の詳細を見る

• Sporamin, a major tuberous root protein of sweet potato, was found to accumulate in large quantities in excised leaves and petioles when such explants were supplied with high concentrations of sucrose. Although a small amount of sporamin could be detected in leaves and petioles treated with 1% or lower concentrations of sucrose, the maximum level of induction required sucrose at a concentration of 3% or higher. The appearance of sporamin polypeptides in leaves and petioles treated with 3% sucrose followed a lag period of about one day, while a significant amount of sporamin mRNAs was already detectable in petioles after one day of treatment with sucrose. Addition of silver nitrate to the medium did not affect the accumulation of sporamin, suggesting that this induction is not due to the effect of ethylene induced by wounding of the tissue. The accumulation of sporamin could also be induced by glucose and by fructose, but not by mannitol, suggesting that changes in carbohydrate and/or energy metabolism in the cell may be involved in the induction. Callus tissues obtained by treatment of leaf segments with 1-naphthaleneacetic acid did not accumulate sporamin even though these cells were cultured on agar medium that contained 3% sucrose. However, when callus tissues were allowed to grow after transfer to a medium that contained 6-benzylaminopurine and sucrose, accumulation of large amounts of sporamin was induced. These results suggest that, while expression of genes coding for sporamin can be induced in organs other than the tuberous root by a process that does not accompany the differentiation of tissue, the induction of expression of sporamin genes by sucrose requires that cells be competent in some specific, but as yet unidentified, way.

• 日本植物生理学会の論文

著者

• NAKAMURA Kenzo

  Laboratory of Biochemistry, Graduate School of Bioagricultural Sciences, Nagoya University

• Hattori Tsukaho

  Laboratory of Biochemistry, School of Agriculture, Nagoya University

• Hattori Tsukaho

  Laboratory Of Biochemistry Faculty Of Agriculture Nagoya University

• Hattori Tsukaho

  Laboratory Of Biochemistry School Of Agriculture Nagoya University

• Fukumoto Harumi

  Laboratory of Biochemistry, School of Agriculture, Nagoya University

• Nakagawa Shoko

  Laboratory of Biochemistry, School of Agriculture, Nagoya University

• Fukumoto Harumi

  Laboratory Of Biochemistry School Of Agriculture Nagoya University

• Nakagawa Shoko

  Laboratory Of Biochemistry School Of Agriculture Nagoya University

• Nakamura Kenzo

  Laboratory Of Biochemistry Faculty Of Agriculture Nagoya University

• Nakamura Kenzo

  Laboratory Of Biochemistry School Of Agriculture Nagoya University

関連論文

• Arabidopsis UPF1 RNA Helicase for Nonsense-mediated mRNA Decay is Involved in Seed Size Control and is Essential for Growth
• TONSOKU is Expressed in S Phase of the Cell Cycle and its Defect Delays Cell Cycle Progression in Arabidopsis
• Spatial Patterns of Sucrose-Inducible and Polygalacturonic Acid-Inducible Expression of Genes That Encode Sporamin and β-Amylase in Sweet Potato : GENE STRUCTURE AND EXPRESSION
• A Family of Potato Genes That Encode Kunitz-Type Proteinase Inhibitors : Structural Comparisons and Differential Expression : GENES STRUCTURE AND EXPRESSION
• Subcellular Localization of the Sweet Potato Tuberous Root Storage Protein(Biological Chemistry)
• Transient Gene Expression in Plant Cells Mediated by Agrobacterium tumefaciens: Application for the Analysis of Virulence Loci
• Transport of a Sweet Potato Storage Protein, Sporamin, to the Vacuole in Yeast Cells
• A Nuclear Factor That Binds to a Dyad-Symmetric Sequence with a CGTCA Motif in the 5'-Upstream Region of the Sweet Potato β-Amylase Gene
• Characterization of a Calcium-Dependent Protein Kinase of Tobacco Leaves That Is Associated with the Plasma Membrane and Is Inducible by Sucrose
• Sucrose-Induced Expression of Genes Coding for the Tuberous Root Storage Protein, Sporamin, of Sweet Potato in Leaves and Petioles
• Tissue-Specific Isoforms of Catalase Subunits in Castor Bean Seedlings
• Different Sets of cis-Elements Contribute to the Expression of a Catalase Gene from Castor Bean during Seed Formation and Postembryonic Development in Transgenic Tobacco : GENE STRUCTURE AND EXPRESSION
• Differential Regulation of the Expression in Transgenic Tobacco of the Gene for β-Glucuronidase under the Control of the 5'-Upstream Regions of Two Catalase Genes from Castor Bean : GENE STRUCTURE AND EXPRESSION
• Structure-Activity Relationships of Jasmonates in the Induction of Expression of Two Proteinase Inhibitor Genes of Potato
• Role of Conserved Residues of the WRKY Domain in the DNA-binding of Tobacco WRKY Family Proteins(Biochemistry & Molecular Biology)
• Expression Patterns of Two Genes for the Delta-Subunit of Mitochondrial F_1-ATP Synthase from Sweet Potato in Transgenic Tobacco Plants and Cells
• A Major Jasmonate-Inducible Protein of Sweet Potato, Ipomoelin, is an ABA-Independent Wound-Inducible Protein
• The N-Terminal Propeptide and the C Terminus of the Precursor to 20-kilo-dalton Potato Tuber Protein Can Function as Different Types of Vacuolar Sorting Signals
• Aspirin and Salicylic Acid Do not Inhibit Methyl Jasmonate-inducible Expression of a Gene for Ornithine Decarboxylase in Tobacco BY-2 Cells
• Construction and Expression in Tobacco of a β-Glucuronidase (GUS) Reporter Gene Containing an Intron Within the Coding Sequence
• An mRNA of Tobacco Cell, Which Is Rapidly Inducible by Methyl Jasmonate in the Presence of Cycloheximide, Codes for a Putative Glycosyltransferase
• The Nuclear Gene for Subunit Vc of Sweet Potato Cytochrome c Oxidase
• Nucleotide Sequence of cDNA for Concanvalin A from Canavalia gladiata Seeds
• The Presequence of a Precursor to the (δ-Subunit of Sweet Poteto Mitochondrial F_1 ATPase is not Sufficient for the Transport of β-Glucuronidase (GUS) into Mitochondria of Tobacco, Rice and Yeast Cells
• Transcript Map of Oppositely Oriented Pea Mitochondrial Genes Encoding the α-Subunit and the Subunit 9 of F_1F_0-ATPase Complex
• Increase in Catalase mRNA in Wounded Sweet Potato Tuberous Root Tissue
• The Presence of Two Forms of Succinate Dehydrogenase in Sweet Potato Root Mitochondria
• A Complete Rat Serum Albumin cDNA Clone Directly Identified by Immunological Screening of cDNA Expression Library(Biological Chemistry)
• A Rapid and Efficient System of Agrobacterium Infection-Mediated Transient Gene Expression in Rice Oc Cells and Its Application for Analysis of the Expression and Antisense Suppression of Preprophytosulfokine, a Precursor of Phytosulfokine-α, Encoded by O
• ACTIVATOR of Spo^::LUC1/WRINKLED1 of Arabidopsis thaliana Transactivates Sugar-inducible Promoters
• Transcription factors for sugar-inducible genes
• Transcription factors for sugar-inducible genes
• Mechanism of the Increase in Succinate Dehydrogenase Activity in Wounded Sweet Potato Root Tissue
• Induction of Polysome Formation in Sweet Potato Root Tissue in Response to Wounding

もっと見る 閉じる

スポンサーリンク