A Complete Rat Serum Albumin cDNA Clone Directly Identified by Immunological Screening of cDNA Expression Library(Biological Chemistry)
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概要
- 論文の詳細を見る
A cDNA expression library was constructed from rat liver poly(A)^+RNA by the vector primer and tailed linker methods using an Escherichia coli expression vector pKEN601. Immunological screening of this library with specific antibody against rat serum albumin identified a colony which carried a plasmid with a cDNA insert of 2.15 kilobases (kb). restriction enzyme mapping and DNA sequencing analyses indicated that this cDNA clone carries a complete length rat serum albumin cDNA, and the 5´-terminus of the cDNA is in the potential capping site of the corresponding nuclear gene sequence.
- 社団法人日本農芸化学会の論文
- 1987-02-23
著者
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NAKAMURA Kenzo
Laboratory of Biochemistry, Graduate School of Bioagricultural Sciences, Nagoya University
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Hattori Tsukaho
Laboratory of Biochemistry, School of Agriculture, Nagoya University
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Hattori Tsukaho
Laboratory Of Biochemistry Faculty Of Agriculture Nagoya University
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Iwatsuki Norio
Koryo International College
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Iwasaki Yukimoto
Laboratory Of Biochemistry Faculty Of Agriculture Nagoya University:(present Office)department Of Bo
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NAKANO Masatoshi
Institute for Medical Science of Aging
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Nakamura Kenzo
Laboratory Of Biochemistry Faculty Of Agriculture Nagoya University
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NAKAMURA Kenzo
Laboratory of Biochemistry, Faculty of Agriculture, Nagoya University
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