Partial Purification and Characterization of Polyphenol Oxidase Isozymes in Banana Bud
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概要
- 論文の詳細を見る
Polyphenol oxidase was extracted from banana buds in the presences of Triton X-100,isoascorbate, and Polyclar AT, and two isozymes I and II have been separated and partially purified by chromatographies on Butyl Toyopearl 650 and DEAE-cellulose. I and II had different mobility in polyacrylamide gel electrophoresis with optimum pHs of 6.8 and 5.5,respectively. Both enzymes showed the apparent K_m values of 0.5 mM for dopamine with substrate inhibitions at its higher concentrations. I and II were inhibited competitively by NaCl with the K_i values of 140 mM and 40 mM, respectively. I and II have a high heat stability, and 88 and 95% of the initial activities were retained after 1-hr incubation at 70℃, respectively.
- 社団法人日本農芸化学会の論文
- 1992-07-23
著者
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Garcia V
Univ. Philippines At Los Banos Laguna Phl
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Oba K
Nagoya Women's Univ. Nagoya Jpn
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Oba Kazuko
Dept. Food Sci. Nutr. Nagoya Women's Univ.
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Oba Kazuko
Laboratory Of Food Biochemistry Nagoya Women's University
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Oba Kazuko
Faculty Of Domestic Science Nagoya University
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Garcia Virgilio
Institute of Food Science and Technology, University of the Philippines at Los Banos, College
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Iwatsuki Norio
Koryo International College
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Uritani I
Laboratory Of Food Biochemistry Nagoya Women's University
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Uritani Ikuzo
Faculty Of Agriculture Nagoya University
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ALVAREZ Angelina
Institute of Food Science and Technology, the University of the Philippines at Los Banos
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Garcia Virgilio
Institute Of Food Science And Technology University Of The Philippines At Los Banos College
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Alvarez Angelina
Institute Of Food Science And Technology The University Of The Philippines At Los Banos
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