Purification and Characterization of a Cd-Binding Complex from the Root Tissue of Water Hyacinth Cultivated in a Cd^<2+>-Containing Medium
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概要
- 論文の詳細を見る
A Cd-binding complex was isolated from water hyacinth root tissue by chromato-graphy with DEAE-cellulose and Sephadex G-50 columns followed by rechromatography on another DEAE-cellulose column. The Cd-binding complex showed a shoulder at 265 nm in the UV absorption spectrum and three bands at 234 (negative), 256 nm (negative), and 277nm (positive) in the CD spectrum. The peptide portion of the complex was composed of only glutamic acid and/or glutamine, cysteine, and glycine in a molar ratio of 10 : 10 : 4. The molecular weight of the complex was 4,000 at neutral pHs but became lower when the complex was treated with mercaptoethanol or acid, suggesting that the complex consists of multiple oligo peptides. These results indicate that water hyacinth root Cd-binding complex is identical to fission yeast Cd-BPl, composed of two each of cadystins A and B.
- 日本植物生理学会の論文
著者
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Fujita Masayuki
Laboratory of Food Hygienics, Faculty of Agriculture, Kagawa University
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Fujita M
Kanazawa Univ. Kanazawa Jpn
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Kawanishi Takahiro
Laboratory of Food Hygienics, Faculty of Agriculture, Kagawa University
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Fujita Masayuki
Laboratory Of Food Hygienics Faculty Of Agriculture Kagawa University
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Kawanishi T
Kagawa Univ. Kagawa Jpn
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Kawanishi Takahiro
Laboratory Of Food Hygienics Faculty Of Agriculture Kagawa University
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Fujita Masayuki
Laboratory Of Biochemistry Faculty Of Agriculture Nagoya University:(present)laboratory Of Food Hygi
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