Kinetic Characterization of Two Phosphate Uptake Systems with Different Affinities in Suspension-Cultured Catharanthus roseus Protoplasts : ENVIRONMENTAL AND STRESS RESPONSES : MEMBRANES AND BIOENERGETICS

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概要

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• We studied the kinetics of inorganic phosphate (P_i) uptake from 0.1-1,000 μM P_i by protoplasts from suspension-cultured cells of Catharanthus roseus (L.) G. Don. Concentration dependence of [^<32>P]P_i uptake revealed two kinetically different uptake systems, a high-affinity system and a low-affinity system, with K_m values of 3.0 and 47 μM, respectively. Protoplasts from cells grown in P_i-rich media had a medium level of the low-affinity activity and a very low level of the high-affinity activity. It appeared low-affinity system is expressed constitutively, while the high-affinity system is regulated by the availability of P_i. When cells grown in a Pi-rich media were transferred to P_i-depleted media, the high-affinity activity increased significantly after 2 d, but the low-affinity activity was barely changed. Upon addition of 10 mM P_i, the high level of the high-affinity activity fell to almost undetectable level in Id. Both uptake systems exhibited maximum activity between pH 5 and 6.

• 日本植物生理学会の論文

著者

• SUZUKI Mikio

  Central Research Laboratory, Hitachi Ltd.

• SUZUKI Mikio

  Department of Molecular and Cell Genetics, School of Life Sciences, Faculty of Medicine, Tottori Uni

• Sakurai Hidehiro

  Department of Biology, School of Education, Waseda University

• Suzuki Masaharu

  Laboratory Of Biochemistry School Of Agriculture Nagoya University

• Furihata Takashi

  Department Of Biology School Of Education Waseda University

• Suzuki M

  Division Of Biological Sciences Graduate School Of Science Hokkaido University

• Furihata T

  Waseda Univ. Tokyo Jpn

• Sakurai Hidehiro

  Department Of Applied Chemistry Graduate School Of Engineering Osaka University

• Sakurai Hidehiro

  Department Of Applied Chemistry Faculty Of Engineering Osaka University

• Suzuki Mikio

  Department Of Molecular And Cell Genetics School Of Life Sciences Faculty Of Medicine Tottori Univer

• Suzuki Mikio

  Department of Applied Chemistry, Faculty of Technology, Gunma University

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