Isozymes of Superoxide Dismutase in Chlamydomonas and Purification of One of the Major Isozymes Containing Fe

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概要

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• Electrophoretic analysis of Chlamydomonas reinhardtii extract revealed at least 4 distinct superoxide dismutase (SOD) activity bands as well as several additional minor bands. Among them, one was deduced to be Fe-type and the other three Mn-type based on their susceptibility to KCN and H_2O_2. The Fe-SOD, which occupied about 40% of the total soluble activity, was purified to homogeneity using ammonium sulfate fractionation followed by DEAE-cellulose, hydroxyapatite, and Superdex 75 gel-permeation chromatography. The 40-kDa native enzyme was composed of two identical 20-kDa subunits with a low shoulder of absorption at 〜350 nm. The NH_2-terminal amino acid sequence determined up to residue 29 showed a high homology to those of Fe-SOD from Arabidopsis thaliana. Glycine max, and Nicotiana plumbagimfolia.

• 日本植物生理学会の論文

著者

• Togasaki R

  Indiana Univ. Indiana Usa

• Sakurai Hidehiro

  Department of Biology, School of Education, Waseda University

• Kusumoto Noriaki

  Sch. Educ. Waseda Univ.

• Kusumoto Noriaki

  Department of Biology, School of Education, Waseda University

• KITAYAMA Kaoru

  Department of Biology, Indiana University

• TOGASAKI Robert

  Department of Biology, Indiana University

• Kitayama Kaoru

  Department Of Biology Indiana University

• Sakurai Hidehiro

  Department Of Applied Chemistry Graduate School Of Engineering Osaka University

• Togasaki Robert

  Department Of Biology Indiana University

• Sakurai Hidehiro

  Department Of Applied Chemistry Faculty Of Engineering Osaka University

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