Pyridoxal 5-phosphate, Phenyl Phosphate and Acetyl Phosphate, as Inhibitors of Photophosphorylation Competitive with Phosphate
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概要
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Pyridoxal 5-phosphate, phenyl phosphate and acetyl phosphate, as well as β-naphthyl monophosphate, inhibited photophosphorylation of spinach chloroplasts competitively with Pi and noncompetitively with ADP. The apparent dissociation constant of the inhibitor-enzyme complex (K_i) values of pyridoxal 5-phosphate, phenyl phosphate and acetyl phosphate for the Pi site were 1.1, 3.8 and 2.4 mM, respectively. These organic phosphates inhibited Ca^<2+>-ATPase of the isolated coupling factor 1 (CF_1) (EC 3.6.1.3) noncompetitively with ATP. AMP, creatine phosphate, fructose 1,6-bisphosphate, glucose 6-phosphate, 3-phosphoglyceric acid, ribose 5-phosphate and PP_i did not significantly inhibit photophosphorylation. Like β-naphthyl monophosphate, pyridoxal 5-phosphate and phenyl phosphate inhibited photophosphorylation and the coupled electron transport, but were almost without effect on the basal electron transport. On the other hand, acetyl phosphate considerably inhibited photophosphorylation, but had almost no effect on the coupled electron transport rate and the basal rate. The results suggest that these organic phosphates inhibit photophosphorylation by binding at the Pi site on the active center of CF_1 and that their binding inhibits the ATPase activity of isolated CF_1. These four organic phosphates which inhibited photophosphorylation competitively with Pi could not substitute for ADP or ATP in inhibiting ferricyanide photoreduction by decreasing H^+-permeability through CF_1 and in protecting the ATPase of isolated CF_1 against cold-anion inactivation.
- 日本植物生理学会の論文
著者
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Sakurai Hidehiro
Department Of Applied Chemistry Graduate School Of Engineering Osaka University
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Shinohara Kenji
Department Of Biology School Of Education Waseda University
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Shinohara Kenji
Department Of Applied Chemistry Graduate School Of Engineering Kyushu University
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Sakurai Hidehiro
Department Of Applied Chemistry Faculty Of Engineering Osaka University
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