Agrobacterium - mediated Transformation of Paulownia (Paulownia fortunei)
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概要
- 論文の詳細を見る
Genetically transformed calli of paulownia (Paulownia fortunei) were generated at high frequency after co-cultivation of petiole segments with Agrobacterium tumefaciens strain LBA4404 that harbored a binary vector (pBI121) which included genes for GUS and NPT II. The effects of acetosyringone, carbon sources and pH on the transformation of paulownia were examined. The presence of acetosyringone in the co-cultivation medium enhanced the induction efficiency of kanamycin-resistant calli. Sucrose was most suitable carbon source in the medium. The pH of the medium had no significant effect on the efficiency at pH 5 to 8. Successful transformation was confirmed by histochemical analysis of GUS activity in kanamycin-resistant calli, and by the detection of NPT II gene in the genome. Kanamycin-resistant calli induced from petiole segments had the ability of regeneration, however, the frequency of regeneration of shoot was very low.
- 日本植物細胞分子生物学会の論文
- 2003-03-03
著者
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Shinohara Kenji
Department of Medicine, Yamaguchi Prefectural Medical Center
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篠原 健司
森林総合研
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MOHRI Takeshi
Department of Molecular and Cell Biology, Forestry and Forest Products Research Institute
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IGASAKI Tomohiro
Department of Molecular and Cell Biology, Forestry and Forest Products Research Institute
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伊ヶ崎 知弘
Department Of Molecular And Cell Biology Forestry And Forest Products Research Institute
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Igasaki Tomohiro
Department Of Molecular And Cell Biology Forestry And Forest Products Research Institute
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Takeshi Mohri
Department Of Molecular And Cell Biology Forestry And Forest Products Research Institute
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Mohri Takeshi
Forestry And Forest Products Research Institute
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Shinohara Kenji
Department Of Biology School Of Education Waseda University
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Igasaki Tomohiro
Department Of Molecular And Cell Biology Forestry And Forest Products Research Institute (ffpri)
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Shinohara Kenji
Department Of Applied Chemistry Graduate School Of Engineering Kyushu University
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