Improvement in the ozone tolerance of poplar plants with an antisense DNA for 1-aminocyclopropane-1-carboxylate synthase
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概要
- 論文の詳細を見る
Genetically transformed lombardy poplar (Populus nigra L. var. italica Koehne) were regenerated after the co-cultivation of stem segments with Agrobacterium tumefaciens strain LBA4404, which harbored a binary vector that included an antisense DNA for ozone-inducible 1-aminocyclopropane-1-carboxylate (ACC) synthase from poplar leaves. Lower rates of ozone-induced ethylene production were observed in transgenic plants than in wild-type plants. Ozone-induced visible damage was attenuated in these lines, and the extent of damage was positively related to the level of ozone-induced ethylene production. In one of these ozone-tolerant lines, the levels of transcripts for ozone-inducible endogenous ACC synthases were suppressed compared with those in wild-type plants, demonstrating that ozone-inducible ACC synthases have a key role in the expression of leaf damage by ozone exposure. Thus, transgenic trees with air pollution tolerance were developed for the first time among woody plants.
- 日本植物細胞分子生物学会の論文
著者
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IGASAKI Tomohiro
Forestry and Forest Products Research Institute
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SHINOHARA Kenji
Forestry and Forest Products Research Institute
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MOHRI Takeshi
Forestry and Forest Products Res. Inst.
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伊ヶ崎 知弘
Department Of Molecular And Cell Biology Forestry And Forest Products Research Institute
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Takeshi Mohri
Department Of Molecular And Cell Biology Forestry And Forest Products Research Institute
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AONO Mitsuko
National Institute for Environmental Studies
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Nakajima Nobuyoshi
National Inst. Environmental Studies Ibaraki Jpn
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Kogawara Satoshi
Forestry and Forest Products Research Institute
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Yasutani Izumi
Toyota Biotechnology & Afforestation Laboratory
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