Agrobacterium-Mediated Transformation of Lombardy Poplar (Populus nigra L. var. italica Koehne) Using Stem Segments

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概要

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• Genetically transformed lombardy poplar (Populus nigra L. var. italica Koehne) plants were regenerated by co-cultivation of stem segments with Agrobacterium tumefaciens strain LBA4404 that harbored a binary vector (pBI121) which included genes for β-glucuronidase (GUS) and neomycin phosphotransferase. Successful transformation was confirmed by the ability of stem segments to produce calli in the presence of kanamycin, histochemical and fluorometric assays of GUS activity in plant tissues, and Southern blot analysis.

• 一般社団法人日本森林学会の論文
• 1996-02-16

著者

• Yamamoto N

  Forestry And Forest Products Research Institute:(present)national Institute Of Agrobiological Resour

• SHINOHARA Kenji

  Forestry and Forest Products Research Institute

• MOHRI Takeshi

  Forestry and Forest Products Res. Inst.

• Yamamoto Naoki

  Forestry and Forest Products Research Institute, Tsukuba Science City

• Mohri T

  Forestry And Forest Products Research Institute

• Shinohara K

  Molecular And Cell Biology Section Bio-resources Technology Division Forestry And Forest Products Re

• Shinohara Kenji

  Forestry And Forest Products Res. Inst.

• Yamamoto Naoki

  Forestry And Forest Products Research Institute

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