Biochemical Studies of a soxF-Encoded Monomeric Flavoprotein Purified from the Green Sulfur Bacterium Chlorobaculum tepidum That Stimulates in Vitro Thiosulfate Oxidation
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概要
- 論文の詳細を見る
In the green sulfur bacterium Chlorobaculum tepidum, three sulfur oxidizing enzyme system (Sox) proteins, SoxAXK, SoxYZ, and SoxB (the core TOMES, thiosulfate oxidizing multi-enzyme system) are essential to in vitro thiosulfate oxidation. We purified monomeric flavoprotein SoxF from this bacterium, which had sulfide dehydrogenase activity. SoxF enhanced the thiosulfate oxidation activity of the purified core TOMES with various cytochromes as electron acceptors to different degrees without any change in the affinity for thiosulfate. The apparent reaction rates with 50 μM- C. tepidum cytochrome c-554 were slightly higher than with horse-heart cytochrome c, and the addition of 0.5 μM- SoxF increased the rate by 92%. The rates with 50 μM- horse-heart cytochrome c and 50 μM- horse-heart cytochrome c plus 0.5 μM- cytochrome c-554 were increased by SoxF by 31% and 120% respectively. We conclude that SoxF mediates electron transfer between the components of core TOMES and externally added cytochromes.
- 社団法人 日本農芸化学会の論文
- 2010-04-23
著者
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Inoue Kazuhito
Department of Biology, School of Education, Waseda University
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Sakurai Hidehiro
Department of Biology, School of Education, Waseda University
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Seo Daisuke
Graduate School Of Science And Technology Kanazawa University
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Inoue Kazuhito
Department Of Biological Sciences Graduate School Of Science University Of Tokyo
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Sakurai Hidehiro
Department Of Applied Chemistry Graduate School Of Engineering Osaka University
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Sakurai Hidehiro
Department Of Biological Sciences Kanagawa University
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Seo Daisuke
Division Of Material Chemistry Graduate School Of Natural Science And Technology Kanazawa University
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OGAWA Takuro
Graduate School of Science, University of Tokyo
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FURUSAWA Toshinari
Graduate School of Science, University of Tokyo
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SHIGA Michiko
Department of Biological Sciences, Kanagawa University
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Ogawa Takuro
Graduate School Of Science University Of Tokyo
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Furusawa Toshinari
Graduate School Of Science University Of Tokyo
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Shiga Michiko
Department Of Biological Sciences Kanagawa University
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Sakurai Hidehiro
Department Of Applied Chemistry Faculty Of Engineering Osaka University
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