Distinct Localization of Peripheral and Central Types of Choline Acetyltransferase in the Rat Cochlea
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概要
- 論文の詳細を見る
We previously discovered a splice variant of choline acetyltransferase (ChAT) mRNA, and designated the variant protein pChAT because of its preferential expression in peripheral neuronal structures. In this study, we examined the immunohistochemical localization of pChAT in rat cochlea and compared the distribution pattern to those of common ChAT (cChAT) and acetylcholinesterase. Some neuronal cell bodies and fibers in the spiral ganglia showed immunoreactivity for pChAT, predominantly the small spiral ganglion cells, indicating outer hair cell type II neurons. In contrast, cChAT- and acetylcholinesterase-positive structures were localized to fibers and not apparent in ganglion cells. After ablation of the cochlear nuclei, many pChAT-positive cochlear nerve fibers became clearly visible, whereas fibers immunopositive for cChAT and acetylcholine esterase disappeared. These results suggested that pChAT and cChAT are localized in different systems of the rat cochlea; pChAT in the afferent and cChAT in the efferent structures.
- 日本組織細胞化学会の論文
著者
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Shimizu Takeshi
Department Of Biochemistry And Genome Biology Hirosaki University Graduate School Of Medicine
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Tooyama Ikuo
Molecular Neuroscience Research Center Shiga University Of Medical Science
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Aimi Yoshinari
Department Of Anatomy Shiga University Of Medical Science Otsu
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Kitano Hiroya
Department Of Otolaryngology Head And Neck Surgery Faculty Of Medicine Tottori University
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Kimura Hiroshi
Molecular Neurobiology Research Center Shiga University Of Medical Science
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Suzuki Mikio
Department Of Molecular And Cell Genetics School Of Life Sciences Faculty Of Medicine Tottori Univer
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Saito Atsushi
Department Of Biochemistry Graduate School Of Biomedical Sciences Hiroshima University
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Aimi Yoshinari
Department of Anatomy, Shiga University of Medical Science
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Kitanishi Tsuyoshi
Molecular Neuroscience Research Center, Shiga University of Medical Science
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Suzuki Mikio
Department of Applied Chemistry, Faculty of Technology, Gunma University
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Kitano Hiroya
Department of Otorhinolaryngology, Head and Neck Surgery, Faculty of Medicine, Tottori University
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