Recombinant Shiga Toxin B Subunit Can Induce Neutralizing Immunoglobulin Y Antibody
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概要
- 論文の詳細を見る
Previously, we have shown that chickens immunized with Shiga toxin (Stx) produce Stx-neutralizing egg yolk immunoglobulin Y (IgY) antibody. The anti-Stx-1 IgY and anti-Stx-2 IgY exert their neutralizing activity through their antibody activity against the B subunit of the toxin but not the A subunit. In the present study, chickens were immunized with recombinant Stx-1 B subunit (rStx-1B) and recombinant Stx-2 B subunit (rStx-2B). Induced anti-rStx-1B and anti-rStx-2B IgY neutralized the toxicity of Stx-1 and Stx-2 against HeLa 229 cells. The neutralizing activity of anti-rStx-1B IgY on Stx-1 was almost 10 times stronger than that of anti-Stx-1 IgY, and that of anti-rStx-2B IgY was 2.6 times stronger than that of anti-Stx-2 IgY. Anti-rStx-1B and anti-rStx-2B IgY reacted with multimeric and monomeric forms of the B subunits in contrast to anti-Stx-1 and anti-Stx-2 IgY that reacted with only the multimeric form. These results indicated that recombinant B subunits were promising antigens for induction of neutralizing antibodies in chickens.
著者
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Shimizu Takeshi
Department Of Biochemistry And Genome Biology Hirosaki University Graduate School Of Medicine
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Tsuji Takao
Department Of Cardiovascular Medicine Okayama University Graduate School Of Medicine And Dentistry
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Kodama Yoshikatsu
Immunology Research Institute Ghen Corporation
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Mori Hiroshi
Microbiology Dep. Of Biopharmaceutical Sciences Gifu Pharmaceutical Univ.
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Mori Hiroshi
Microbiology, Department of Biopharmaceutical Sciences, Gifu Pharmaceutical University
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Neri Paola
Microbiology, Department of Biopharmaceutical Sciences, Gifu Pharmaceutical University
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Sugiyama Tsuyoshi
Microbiology, Department of Biopharmaceutical Sciences, Gifu Pharmaceutical University
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Tokoro Shunji
Microbiology, Department of Biopharmaceutical Sciences, Gifu Pharmaceutical University
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Umeda Kouji
Immunology Research Institute, GHEN Corporation
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