Protective eff ect of intranasal vaccination with nontoxic mutant TSST-1 against Staphylococcus aureus infection
スポンサーリンク
概要
- 論文の詳細を見る
Infection caused by methicillin-resistant Staphylococcus aureus (MRSA) has been the most commonlyacquired types of nosocomial infections. It was reported that anterior nares are the major reservoir of S. aureus andthe source of 80% of S. aureus bacteremia is endogenous. Considering these facts, elimination and reduction of nasalcarriage are thought to be eff ective protection against systemic S. aureus infection and nosocomial infection. Toxic shocksyndrome toxin 1( TSST-1) is one of superantigens secreted by S. aureus. Previously, it was reported that mutant form(H135A) of TSST-1( mTSST-1) was shown to be nontoxic, and subcutaneous vaccination with mTSST-1 could protectagainst systemic S. aureus infection in a mouse model. In this study, we investigated the protective eff ect of intranasalvaccination with mTSST-1 supplemented with non-toxic mutant( H44A) Escherichia coli heat labile toxin( mLT) as amucosal adjuvant. The results demonstrated that intranasal immunization with mTSST-1 plus mLT could efficientlyinduce production of anti-TSST-1 antibodies in sera and also induce anti-TSST-1 IgA production in bronchoalveolar lavagefl uids( BALF) of vaccinated mice. In nasal-associated lymphoid tissues( NALT) of vaccinated mice, anti-TSST-1 IgAsecreting cells were signifi cantly increased. To evaluate of the protective eff ect of this vaccine against systemic S. aureusinfection, BALB/c mice were vaccinated with mTSST-1 plus mLT and challenged with clinical isolated S. aureus 834intravenously. Bacterial numbers in spleen and liver, and cumulative mortality rate of vaccinated mice were lower thanthose of control mice. We further developed a mouse model of nasal S. aureus colonization. S. aureus bacterial numbers innasal cavity of vaccinated mice were signifi cantly reduced compared with those of control mice. These results indicatethat intranasal immunization with mTSST-1 plus mLT is able to induce systemic and mucous immune responses and ofprovide protection against systemic S. aureus infection and nasal colonization.
- 弘前大学の論文
著者
-
Nakane Akio
Department of Microbiology and Immunology, Hirosaki University Graduate School of Medicine
-
HU Dong-Liang
Department of Microbiology and Immunology, Hirosaki University Graduate School of Medicine
-
Nakane Akio
Department Of Microbiology And Immunology Hirosaki University Graduate School Of Medicine
-
Tsuji Takao
Department Of Medicine And Medical Science Okayama University Graduate School Of Medicine And Dentis
-
Nakane Akio
Department Of Bacteriology Hirosaki University School Of Medicine
-
Narita Kouji
Department of Microbiology and Immunology, Hirosaki University Graduate School of Medicine
-
Hu Dong-liang
Department Of Microbiology And Immunology Hirosaki University Graduate School Of Medicine
-
Tsuji Takao
Department Of Cardiovascular Medicine Okayama University Graduate School Of Medicine And Dentistry
-
Narita Kouji
Department Of Microbiology And Immunology Hirosaki University Graduate School Of Medicine
-
Tsuji Takao
Department Of Microbiology Fujita Health University School Of Medicine
関連論文
- <Poster>Mouse peptidoglycan recognition protein( PGRP)-S : the role in bacterial infection
- <Poster>The role of interleukin-17 in the protective effect of an immunization with clumping factor A against Staphylococcus aureus infection
- <Poster>Immunization with nontoxic mutant staphylococcal enterotoxin a protects against enterotoxin-induced emesis in house musk shrews
- TNF-αとIFN-γはCorynebacterium pseudotuberculosis再感染マウスの防御免疫の成立に必要である
- 黄色ブドウ球菌に対するワクチンの開発 (特集 感染症に対するワクチンの開発とその免疫理論)
- 肥満による感染防御能の低下
- 無毒変異TSST-1経鼻粘膜ワクチンによる黄色ブドウ球菌感染防御効果
- サケ軟骨プロテオグリカンが加熱死菌刺激マウスマクロファージの反応に及ぼす影響の解析
- 黄色ブドウ球菌腸管毒素とT細胞レセプターVβエレメントとの結合の解析
- ブドウ球菌エンテロトキシンAの催吐メカニズムとその制御機構
- プラスミドpF5のブドウ球菌エンテロトキシン様毒素遺伝子近傍領域の解析
- ブドウ球菌エンテロトキシン保有プラスミドにコードされる新規スーパー抗原性毒素様遺伝子の解析
- ビタミンA活性化誘導体atRAのリステリア感染に対する宿主抵抗性増強効果
- 日本と中国における急性胃腸炎起因微生物の汚染調査
- 腹部X線照射によるマウス腸管障害モデルの作製とヘパラン硫酸の影響
- 微生物学の進歩 レプチンと感染免疫
- マウスX線全身照射による放射線腸障害にEGF, メラトニン, エダラボンの影響
- MIFの生物活性
- ブドウ球菌エンテロトキシンAの嘔吐活性に関する中和エピトープの解析
- 合成ペプチドを利用したブドウ球菌エンテロトキシンA分子上のエピトープの同定
- 腸管粘膜免疫と常在細菌 (特集 粘膜免疫の特性と制御)
- 話題 活性化受容体TREM-1は敗血症ショックの病態形成に重要である
- 第73回 日本細菌学会総会
- All-trans-retinoic acid (atRA) increases host resistance to Listeria monocytogenes infection
- Retinoic acid-inducible gene-I( RIG-I) and diseases
- Transplantation of Mesenchymal Stem Cells to Prevent Radiation-induced Intestinal Injury in Mice
- Hypoxia Expression in Radiation-induced Late Rectal Injury
- Nuclear Accumulation and Activation of Nuclear Factor κB after Split-dose Irradiation in LS174T Cells
- ブドウ球菌エンテロトキシンAの催吐メカニズムとその制御機構
- Basement Membrane Type IV Collagen Molecules in the Choroid Plexus, Pia Mater and Capillaries in the Mouse Brain
- Listeria monocytogenes Induces the Expression of Retinoic Acid-Inducible Gene-I
- リステリア感染におけるペプチドグリカン認識蛋白質の役割--マウスとショウジョウバエの研究からみえること (特集 感染防御とパターン認識レセプター)
- Urocortin 2 suppresses host resistance to Listeria monocytogenes infection via up-regulation of IL-10
- Sequential Involvement of NK Cells and CD8^+ T Cells in Granuloma Formation of Rhodococcus aurantiacus-Infected Mice
- CD3^+/TCR-αβ^- Cells Are Important in Protecting Spinal Cord Tissues against Theiler's Virus Strain GD VII Infection
- Systemic Dissemination by Intrarectal Infection with Listeria monocytogenes in Mice
- Inhibition of Transforming Growth Factor-β, Hypoxia-inducible Factor-1α and Vascular Endothelial Growth Factor Reduced Late Rectal Injury Induced by Irradiation
- Colonization and Differentiation of Transplanted Embryonic Stem Cells in the Irradiated Intestine of Mice
- Staphylococcal enterotoxin a modulates intracellular Ca^2+ signal pathway in human intestinal epithelial cells
- 153 Radioprotection by Edaravone Reagents against Radiation Intestinal Injury(Radio-protection and sensitization, Abstracts of the 46th Annual Meeting of the Japan Radiation Research Society)
- <Poster>Cystatin C immunoreactivity and neuronal degeneration in amyotrophic lateral sclerosis
- Protective eff ect of intranasal vaccination with nontoxic mutant TSST-1 against Staphylococcus aureus infection
- Vaccination with a nontoxic mutant of staphylococcal enterotoxin C elicits Th2 immune response for protection against Staphylococcus aureus infection
- Complement Receptor Type 3 Plays an Important Role in Development of Protective Immunity to Primary and Secondary Corynebacterium pseudotuberculosis Infection in Mice
- Role of Endogenous Tumor Necrosis Factor Alpha and Gamma interferon in Resistance to Corynebacterium pseudotuberculosis infection in Mice
- Salmon cartilage proteoglycan modulates cytokine responses to Escherichia coli in mouse macrophages
- Enzyme-Linked Immunoassay for Midkine, and Its Application to Evaluation of Midkine Levels in Developing Mouse Brain and Sera from Patients with Hepatocellular Carcinomas
- C Terminal Half Fragment (50kDa) of Heavy Chain Components of Clostridium botulinum Type C and D Neurotoxins Can Be Used as an Effective Vaccine
- Exponential Hyperbolic Sine Function Fitting of Heart Rate Response to Constant Load Exercise
- New Inuyama classification; new criteria for histological assessment of chronic hepatitis^1
- Spatial changes of gelatinolytic activity in myocardial infarction in rats
- High serum Brain Natriuretic peptide levels as a marker of risk for silent stroke in patient with non-valvular Af
- A monoclonal antibody to rat asialoglycoprotein receptor that recognizes an epitope specific to its major subunit
- CONCURRENT GASTRIC AND COLONIC LOW-GRADE MUCOSA-ASSOCIATED LYMPHOID TISSUE LYMPHOMATA IN A PATIENT WITHOUT HELICOBACTER PYLORI INFECTION
- Nicorandil decreased infarct size with enhancement of Serum interleukin 6 levels in patients with acute myocardial infarction after successful reperfusion
- A Hemolysin of Vibrio mimicus (VMH) Stimulates Cells to Produce ATP and Cyclic AMP Which Appear to Be Secretory Mediators
- Comparison of a Reversed Passive Latex Agglutination and a Polymerase Chain Reaction for ldentification of Cholera Toxin Producing Vibrio cholerae O1
- Monomer of the B Subunit of Heat-Labile Enterotoxin from Enterotoxigenic Escherichia coli Has Little Ability to Bind to GM_1 Ganglioside Compared to Its Coligenoid
- Rapid increase of matrix adhesive glyocprotein, thrombospondin-1 (TSP-1) mRNA in rat myocardial infarction (MI) and its localization
- Heparin Binding Epidermal Growth Factor-like Growth Factor (HB-EGF) is Increased in Myocardial Infarction (MI) : Enhancement by Reperfusion and Auto-induction by Recombinant Protein
- Endothelial Cells of Newly Formed Vessels Express Osteonectin mRNAs in the Border Zone of Myocardial Infarction (MI) in Rats
- Chemokine Receptor CXCR-3 Was Up-regulated in Rat Myocardial Infarction(MI) : Anasysis of cDNA Array and Quantitative Expression by Real Time PCR
- Tissue Inhibitor of Metalloproteinase-2 Localization in Myocardial Infarction (MI) in Rats : Another Role as an Activator for Metalloproteinase-2
- Clinicopathological features of acute-onset autoimmune hepatitis
- A scoring system for primary biliary cirrhosis and its application for variant forms of autoimmune liver disease
- Detection of hepatitis B virus DNA in the liver and serum of patients with hepatitis B surface antigen and hepatitis C virus antibody negative chronic liver disease
- Natural Killer (NK)-Like Cytotoxicity of Murine Intraepithelial Lymphocytes in the Small Intestine (iIEL) and the Effect of the Serine Proteases
- Quantitative analysis of wild-type and precore mutant hepatitis B virus in carriers
- Inhibition of mucosal and systemic T_h2-type immune responses by intranasal peptides containing a dominant T cell epitope of the allergen Der p 1
- The Oldest Patient with Gallstone Ileus: Report of a Case and Review of 176 Cases in Japan
- レプチンと感染免疫
- Superantigenic Toxin Genes Coexist with Specific Staphylococcal Cassette Chromosome mec Genes in Methicillin-Resistant Staphylococcus aureus
- Clinical Evaluation of Intramuscular Administration of Natural Interferon-γ in the Treatment of Chronic Hepatitis B
- Recombinant Shiga Toxin B Subunit Can Induce Neutralizing Immunoglobulin Y Antibody
- Comparative Prevalence of Antibiotic Resistance, Staphylococcal Cassette Chromosome Mec and Superantigenic Toxin Genes in Coagulase-Negative Staphylococci
- Transplantation of Mesenchymal Stem Cells to Prevent Radiation-induced Intestinal Injury in Mice
- Reduced IL-1β Production in Diet-Induced Obese Mice Impairs Host Defense against Skin Staphylococcus Aureus Infection
- Adipose Tissue-Derived Mesenchymal Stem Cells Attenuate Fulminant Hepatitis in Mice Induced by Concanavalin A
- Analysis of the Epitopes on Staphylococcal Enterotoxin A Responsible for Emetic Activity.
- OPC-8212, a Quinoline Derivative, Counteracts the Reduction in Type III Collagen mRNA due to Lipopolysaccharides in Cultured Rat Cardiac Fibroblasts.
- Enzyme-Linked Immunoassay for Midkine, and Its Application to Evaluation of Midkine Levels in Developing Mouse Brain and Sera from Patients with Hepatocellular Carcinomas.