無毒変異TSST-1経鼻粘膜ワクチンによる黄色ブドウ球菌感染防御効果

概要

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Toxic shock syndrome toxin 1 (TSST-l) produced by Staphylococcus aureus is one of superantigenicexotoxins and is best known for its involvement in toxic shock syndrome. To investigate whether an intranasalvaccination with a nontoxic mutant TSST-1 (mTSST-l) can protect against systemic infection and nasal colonizationof S. aureus, mice were immunized with mTSST-1 plus a nontoxic mutant heat labile toxin (mLT) of Escherichiacoli. After an intravenous challenge with S. aureus. bacterial growth in the organs of immunized mice reduced andthe survival rate was significantly higher, compared with the naive mice. To evaluate the effect of vaccination withmTSST-l against nasal S. aureus colonization. mice were challenged with S. aureus intranasally. The bacterial countsin the nasal cavity of immunized mice were significantly reduced. Vaccination with mTSST-l plus mLT efficientlyinduced anti-TSST-l antibodies in the sera and mucosal exudates. and increased anti-TSST-l IgA-secreting cells innasal-associated lymphoid tissues. These results indicate that an intranasal vaccination with mTSST-l plus mLTcould induce systemic and mucosal immune responses and provide protection against not only systemic infection butalso nasal colonization of S. aureus.
弘前大学の論文