Nuclear Accumulation and Activation of Nuclear Factor κB after Split-dose Irradiation in LS174T Cells

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概要

• 論文の詳細を見る

• Although radiation-induced gene expression has been extensively studied, most of the studies to date have focused on that after single-dose irradiation. As split-dose irradiation, rather than single-dose irradiation, is usual in clinical situations, we investigated the effects of split-dose irradiation on nuclear factor κB (NF-κB) in the human rectum carcinoma cell line, LS174T. After either single- or split-dose irradiation with a different interval, nuclear localization of NF-κB was examined by Western blot and immunofluorescence and its DNA-binding activity was measured by ELISA-based assay. Irradiation-induced NF-κB nuclear accumulation and DNA binding activity increased in a dose-dependent manner. The peak of NF-κB nuclear accumulation and DNA binding activity was seen 2 to 6 hours after a single dose of 4 Gy irradiation and returned to control levels after 12 hours. In split-dose irradiation, NF-κB activity was similar after the first and second doses of 4 Gy irradiation separated by 12 hours. In addition, NF-κB activity was decreased by lengthening the interval between irradiation. The cell survival, which was assessed by colony formation assay, showed inverse correlation to this: the surviving fraction was higher after split-dose irradiation than after single-dose irradiation of the same total dose and it increased as the interval between irradiation was lengthened. Thus the present results showed a correlation between NF-κB activation and the repair of sublethal damage in split-dose irradiation.

• 日本放射線影響学会の論文
• 2007-01-15

著者

• Nakane Akio

  Department of Microbiology and Immunology, Hirosaki University Graduate School of Medicine

• Miyakoshi Junji

  Department of Radiation Genetics,Graduate School of Medicine Kyoto University

• Liu Yong

  Particle Radiation Oncology Research Center Kyoto University

• Liu Yong

  Particle Radiation Oncology Research Center Research Reactor Institute Kyoto University

• HU Dong-Liang

  Department of Microbiology and Immunology, Hirosaki University Graduate School of Medicine

• LIU Yong

  Departments of Radiology and Radiation Oncology, Hirosaki University Graduate School of Medicine

• ABE Yoshinao

  Departments of Radiology and Radiation Oncology, Hirosaki University Graduate School of Medicine

• NAKAHARA Takehisa

  Department of Radiological Technology, School of Health Science, Faculty of Medicine, Hirosaki Unive

• Nakane Akio

  Department Of Microbiology And Immunology Hirosaki University Graduate School Of Medicine

• Liu Yong

  Department Of Applied Chemistry School Of Engineering University Of Toyama

• Nakane Akio

  Department Of Bacteriology Hirosaki University School Of Medicine

• Miyakoshi Junji

  Department Of Radiological Technology School Of Health Science Faculty Of Medicine Hirosaki Universi

• Hu Dong-liang

  Department Of Microbiology And Immunology Hirosaki University Graduate School Of Medicine

• Miyakoshi Junji

  Department Of Radiation Biology Kyoto College Of Pharmacy

• Abe Yoshinao

  Department Of Radiology And Radiation Oncology Hirosaki University

• Abe Yoshinao

  Department Of Nuclear Medicine And Radiology Division Of Brain Sciences Institute Of Development Agi

• Nakahara Takehisa

  Department Of Radiological Technology School Of Health Science Faculty Of Medicine Hirosaki Universi

• Liu Yong

  Department Of Anatomy School Of Medicine Xi'an Jiaotong University

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