Combined Exposure of ELF Magnetic Fields and X-rays Increased Mutant Yields Compared with X-rays Alone in pTN89 Plasmids
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概要
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We have examined mutations in the supF gene carried by pTN89 plasmids in Escherichia coli (E. coli) to examine the effects of extremely low frequency magnetic fields (ELFMFs) and/or X-rays to the plasmids. The plasmids were subjected to sham exposure or exposed to an ELFMF (5mT), with or without X-ray irradiation (10Gy). For the combined treatments, exposure to the ELFMF was immediately before or after X-ray irradiation. The mutant fractions were 0.94×10^<-5> for X-rays alone, 1.58×10^<-5> for an ELFMF followed by X-rays, and 3.64×10^<-5> for X-rays followed by an ELFMF. Increased mutant fraction was not detected following exposure to a magnetic field alone, or after sham exposure. The mutant fraction for X-rays followed by an ELFMF was significantly higher than those of other treatments. Sequence analysis of the supF mutant plasmids revealed that base substitutions were dominant on exposure to X-rays alone and X-rays plus an ELFMF. Several types of deletions were detected in only the combined treatments, but not with X-rays alone. We could not find any mutant colonies in sham irradiated and an ELFMF alone treatment, but exposure to ELFMFs immediately before or after X-ray irradiation may enhance the mutations. Our results indicate that an ELFMF increases mutation and alters the spectrum of mutations.
- 日本放射線影響学会の論文
- 2005-06-30
著者
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Miyakoshi Junji
Department of Radiation Genetics,Graduate School of Medicine Kyoto University
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KOYAMA Shin
Department of Radiological Technology, School of Health Sciences, Faculty of Medicine, Hirosaki Univ
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SAKURAI Tomonori
Department of Radiological Technology, School of Health Sciences, Faculty of Medicine, Hirosaki Univ
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NAKAHARA Takehisa
Department of Radiological Technology, School of Health Science, Faculty of Medicine, Hirosaki Unive
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Miyakoshi Junji
Dep. Of Radiological Life Sciences Div. Of Medical Life Sciences Graduate School Of Health Sciences
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Miyakoshi Junji
Department Of Radiation Genetics Faculty Of Medicine Kyoto University
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KOMATSUBARA Yoshiki
Department of Radiological Technology, School of Health Sciences, Faculty of Medicine, Hirosaki Univ
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ISOZUMI Yasuhito
Department of Interdisciplinary Environment, Graduate School of Human and Environmental Studies, Kyo
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Komatsubara Yoshiki
Department Of Radiological Technology School Of Health Sciences Faculty Of Medicine Hirosaki Univers
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Koyama Shin
Department Of Radiological Technology School Of Health Sciences Faculty Of Medicine Hirosaki Univers
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Miyakoshi Junji
Department Radiation Genetics Graduate School Of Medicine Kyoto University
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Miyakoshi Junji
Department Of Radiation Biology Kyoto College Of Pharmacy
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Miyakoshi Junji
Faculty Of Medicine Hirosaki University
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Sakurai Tomonori
Laboratory Of Applied Radio Engineering For Humanosphere Research Institute For Sustainable Humanosp
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Sakurai Tomonori
Department Of Radiological Technology School Of Health Sciences Faculty Of Medicine Hirosaki Univers
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Nakahara Takehisa
Department Of Radiological Technology School Of Health Sciences Faculty Of Medicine Hirosaki Univers
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Nakahara Takehisa
Department Of Radiological Technology School Of Health Science Faculty Of Medicine Hirosaki Universi
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Isozumi Yasuhito
Department Of Interdisciplinary Environment Graduate School Of Human And Environmental Studies Kyoto
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