Radiation-induced Reduction of Osteoblast Differentiation in C2C12 cells
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概要
- 論文の詳細を見る
Therapeutic radiation causes bone damage and may increase fracture risks in treatment for head-and-neck cancer and in pelvic irradiation. These properties can also be used for prevention of heterotopic ossification in hip arthroplasty. To evaluate the effects of ionizing radiation on osteoblast differentiation, C2C12 cells were directed into an osteogenic lineage by treatment with a combination of bone morphogenic protein 2 (BMP-2) (100 ng/ml) and heparin (30 μg/ml) 6 h after irradiation (2 and 4 Gy). Osteoblast differentiation was evaluated based on alkali phosphatase (ALP) activity and expression of mRNA encoding ALP and collagen type I. Ionizing radiation suppressed the growth of C2C12 cells and decreased expression of ALP and collagen type I mRNAs with concomitant reduction of the ALP activity. Although further studies are needed to elucidate the molecular mechanism, our findings suggest that ionizing radiation at therapeutic doses interferes with bone formation by reducing ALP activity and expression of mRNA encoding ALP and collagen type I.
- 日本放射線影響学会の論文
- 2007-11-15
著者
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Miyakoshi Junji
Department of Radiation Genetics,Graduate School of Medicine Kyoto University
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SAKURAI Tomonori
Department of Radiological Technology, School of Health Sciences, Faculty of Medicine, Hirosaki Univ
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Miyakoshi Junji
Department Of Radiological Technology School Of Health Sciences Faculty Of Medicine Hirosaki Univers
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Miyakoshi Junji
Department Of Radiological Life Sciences Division Of Medical Life Sciences Graduate School Of Health
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SAWADA Yohei
Department of Radiological Technology, School of Health Sciences, Faculty of Medicine, Hirosaki Univ
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YOSHIMOTO Miwa
Department of Radiological Technology, School of Health Sciences, Faculty of Medicine, Hirosaki Univ
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KAWAI Miyuki
Department of Radiological Technology, School of Health Sciences, Faculty of Medicine, Hirosaki Univ
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Miyakoshi Junji
Department Radiation Genetics Graduate School Of Medicine Kyoto University
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Miyakoshi Junji
Department Of Radiation Biology Kyoto College Of Pharmacy
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Miyakoshi Junji
Faculty Of Medicine Hirosaki University
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Sawada Yohei
Department Of Radiological Technology School Of Health Sciences Faculty Of Medicine Hirosaki Univers
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Kawai Miyuki
Department Of Radiological Technology School Of Health Sciences Faculty Of Medicine Hirosaki Univers
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Yoshimoto Miwa
Department Of Radiological Technology School Of Health Sciences Faculty Of Medicine Hirosaki Univers
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Sakurai Tomonori
Department Of Radiological Technology School Of Health Sciences Faculty Of Medicine Hirosaki Univers
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