Radiosensitization by Overexpression of the Nonphosphorylation Form of IκB-α in Human Glioma Cells
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概要
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To assess the role of NF-κB in cellular radiosensitivity, we constructed mutated IκB expression plasmids for SY-IκB (with mutations at residues of 32, 36 and 42) expression in human malignant glioma cells (radiosensitive MO54 and radioresistant T98 cells), giving respective cell types referred to as MO54-SY4 and T98-SY14. Both of the clones expressing SY-IκB became radiosensitive, compared with the parental MO54 and T98 cells. A treatment with herbimycin A or genistein did not change the radiosensitivity of cells expressing SY-IκB, but made both the MO54 and T98 parental cells more sensitive to ionizing radiation. A treatment with TNF-α induced DNA fragmentation and apoptosis in cells expressing SY-IκB, but not in MO54 and T98 cells. The survival after X-ray exposure of the parental MO54 cells was slightly increased by a TNF-α treatment, but that of the parental T98 cells did not change. The change in sensitivity to ultra-violet (UV) radiation and adriamycin in MO54-SY4 cells was very similar to that for X-ray sensitivity, but no change was observed in T98-SY14 cells. Significant sublethal damage repair was observed in T98 cells, whereas MO54 cells showed little repair activity. The expression of p53 was enhanced in the parental MO54 cells, while the p53 levels in the MO54-SY4, and in the parent and clonal T98 cells, did not change. Our data suggest that the serine and tyrosine phosphorylation of IκB-α may play a role in determining the radiosensitivity of malignant glioma cells.
- 日本放射線影響学会の論文
著者
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Miyakoshi Junji
Department of Radiation Genetics,Graduate School of Medicine Kyoto University
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Miyakoshi Junji
Department Of Radiation Genetics Faculty Of Medicine Kyoto University
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Honda Naoko
Department of Mechanical Science and Engineering, Nagoya University
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Yagi Kasumi
京都大学 医学研究科 放射線遺伝学
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Yagi Kasumi
Department Of Zoology Graduate School Of Science Kyoto University
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Yagi Kasumi
Department Radiation Genetics Graduate School Of Medicine Kyoto University:department Of Zoology Gra
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Honda Naoko
Department Radiation Genetics Graduate School Of Medicine Kyoto University
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Ding Gui-rong
Department Radiation Genetics Graduate School Of Medicine Kyoto University:department Of Radiation M
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Miyakoshi Junji
Department Radiation Genetics Graduate School Of Medicine Kyoto University
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Miyakoshi Junji
Department Of Radiation Biology Kyoto College Of Pharmacy
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Miyakoshi Junji
Faculty Of Medicine Hirosaki University
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Honda Naoko
Department Of Integrated Biosciences Graduate School Of Frontier Sciences The University Of Tokyo
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