Staphylococcal enterotoxin a modulates intracellular Ca^2+ signal pathway in human intestinal epithelial cells

概要

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To clarify whether SEA can aff ect any changes of cellular signalling pathways in intestinal epithelial cells, weperformed experiments to see if SEA can modulate the intracellular signaling pathway in intestinal epithelial cells. Wedemonstrate here that SEA induces an increase in intracellular calcium（［Ca2+］i） in human intestinal epithelial cells andthe［ Ca2+］i is released from intracellular stores. SEA-induced increase of［ Ca2+］i was clearly inhibited by treatment witha nitric oxide synthase（ NOS） inhibitor, NG-monomethyl-L-arginine. Intestinal epithelial cells express endothelial NOS（eNOS） in resting cell condition, and express inducible NOS（ iNOS） after stimulating with tumor necrosis factor（ TNF）-α. TNF-α-pretreated cells showed a signifi cant increase in［ Ca2+］i that was also inhibited by the NOS inhibitor. Theseresults demonstrate that SEA induces an increase in intracellular Ca2+ concentration in human intestinal epithelial cells,and the expressions of both eNOS and iNOS could be responsible for the increase in intracellular Ca2+ concentration inthe cells induced by SEA. It is important to study the relationship of NOS expression, Ca2+ signal pathway and emesisfor understanding the mechanism of SEA-induced food poisoning.
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