＜Poster＞The role of interleukin-17 in the protective effect of an immunization with clumping factor A against Staphylococcus aureus infection

概要

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Clumping factor A （ClfA） is an adhesin of Staphylococcus aureus, and the residues 40-559 of ClfA （ClfA40-559） compose fi brinogen binding domain. It was reported that antibody to ClfA40-559 plays an important role in theprotective eff ects. In this study, we investigated the role of IL-17 in the protective eff ects of an immunization withClfA40-559 against S. aureus infection.　Mice immunized or non-immunized with ClfA40-559 were challenged with S. aureus. Bacterial numbers in the organsand survival rates were evaluated. RAW264.7, DC2.4 and spleen cells of naïve and immunized mice were stimulatedwith ClfA40-559. Cytokine production and mRNA expression in the spleens and cells was determined by ELISA and/orRT-PCR.　 The survival rate was improved and the bacterial numbers in the organs were reduced in the mice immunizedwith ClfA40-559. ClfA40-559 induced IL-23p19 mRNA expression and IL-6 but not IL-12 production in DC2.4 andRAW264.7 cells. RORγt and IL-17A mRNA expression in the spleen cells of naïve mice was induced. In the spleencells of immunized mice, ClfA40-559 induced the high production of IL-17 but not IFN-γ or IL-4. IL-17A, IL-6 and CXCL2mRNA expression was also increased in the organs of immunized and challenged mice. These results suggest thatan immunization with ClfA40-559 induces Th17, and that IL-17 contributes to host defense in early phase of S. aureusinfection by promoting neutrophil recruitment.