cDNA Cloning of a Wounding-Inducible Gene Encoding a Plastid ω-3 Fatty Acid Desaturase from Tobacco

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概要

• 論文の詳細を見る

• A cDNA encoding the plastid ω-3 fatty acid desatura-se was isolated from a tobacco (Nicotiana tabacum cv. SR1) leaf cDNA library. The amino terminal extension of the deduced amino acid sequence of this clone had a characteristic feature of the transit peptides of plastid-destined proteins. Northern analysis indicated that the mRNA corresponding to this cDNA was present in leaves, but was not detected in roots. Responses to wounding of the plastid and microsome ω-3 desaturase genes were investigated in tobacco leaves. The mRNA level of the plastid ω-3 desatu-rase gene increased to about 2-fold that of unwounded controls at 12 h after a wounding treatment. On the other hand, the mRNA level of the microsome ω-3 desaturase gene remained constant in the wounded leaves. Linolenic acid contents of major leaf polar lipids increased by wounding. These results indicate that wounding enhances the accumulation of the plastid ω-3 desaturase mRNA, and incteases the conversion of linoleic acid to linolenic acid in leaf polar lipids.

• 日本植物生理学会の論文

著者

• Nishiuchi Takumi

  Dept. Biol. Fac. Sci. Kyushu Univ.

• Nishiuchi Takumi

  Laboratory For Remediation Research Plant Science Center Riken:institute For Gene Research Kanazawa

• Nishiuchi Takumi

  Department Of Biology Faculty Of Science Kyushu University

• KODAMA Hiroaki

  Department of Chemistry, Faculty of Science and Engineering, Saga University

• Kodama H

  Department Of Chemistry Faculty Of Science And Engineering Saga University

• Kodama Hiroaki

  Dept. Biore. Chem. Fac. Horti. Chiba Univ.

• IBA Koh

  Department of Biology, Faculty of Science, Kyushu University

• Iba Koh

  Dept. Biol. Grad. Sch. Sci. Kyushu Univ.

• Iba Koh

  Department Of Biology Faculty Of Science Kyushu University

• Kodama Hiroaki

  東京理科大学 薬学部分子病態学

• HAMADA Tatsurou

  Ishikawa Agri. Coll.

• Hamada Tatsurou

  Department of Biology, Faculty of Science, Kyushu University

• Nishimura Mitsuo

  Department of Biology, Faculty of Science, Kyushu University

• Kodama Hiroaki

  Department Of Anatomy Ohu University School Of Dentistry

• Nishimura M

  Dept. Cell Biol. Nat. Inst. Basic Biol.

• Nishimura Mitsuo

  Departmcnt Of Biology Faculty Of Science Kyushu University

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