Molecular Cloning and Allergenicity of Pen j 1, a Major Allergen of Kuruma Prawn, Penaeus japonicus
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概要
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Tropomyosins have been identified as a common allergen in crustaceans, but their allergenicity is not well understood. In the present study, we isolated an allergen, Pen j 1, a tropomyosin from kuruma prawn Penaeus japonicus, and determined its N-terminal amino acid sequence. The cDNA encoding the allergen was cloned by 5′- and 3′-rapid amplification of cDNA ends (RACE), and was found to code for a protein which consists of 284 amino acid residues. Sequencing analyses indicated for the first time that mature tropomyosin is formed by the elimination of a leader peptide of nine amino acid residues. To elucidate the binding sites of IgE antibodies in the sera of shrimp-sensitive patients, various recombinant peptides were expressed in Escherichia coli as fusion proteins with glutathione S-transferase (GST), and the examined with regard to reactivity with IgE antibodies. The IgE-binding epitopes were found to locate over the whole sequence of the allergen, and the IgE antibodies in the sera were found to recognize strongly its C-terminal region.
著者
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HIEMORI Miki
Department of Nutritional Science, Okayama Prefectural University
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YAMASHITA Hiromi
Department of Nutritional Science, Okayama Prefectural University
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TSUJI Hideaki
Department of Nutritional Science, Okayama Prefectural University
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SAKAI KENTARO
Department of Molecular Science and Material Engineering, Graduate School of Science and Technology,
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MATSUMOTO Tomoaki
Department of Humanity and Environment, Hosei University
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Kimoto Masumi
Department Of Nutritional Science Faculty Of Health And Welfare Okayama Prefectural University
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KUNIMOTO Ayumi
Department of Nutritional Science, Faculty of Health and Welfare Science, Okayama Prefectural Univer
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TAKAHASHI Kyoko
Department of Food Science and Nutrition, School of Human Environmental Sciences, Mukogawa Women’s U
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SISINO Takako
Department of Nutritional Science, Faculty of Health and Welfare Science, Okayama Prefectural Univer
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