Development of a Method for the Determination of γ-Aminobutyric Acid in Foodstuffs

スポンサーリンク

概要

• 論文の詳細を見る

• A method for the determination of γ-aminobutyric acid (GABA) in foodstuffs has been developed by combination of its dinitrophenylation and high-performance liquid chromatography (HPLC) using norleucine as an internal standard. GABA was converted to its stable derivative with 1-fluoro-2,4-dinitrobenzene and the derivative was extracted with ether. After evaporation of the extract, the residue was dissolved in 0.1 M NaOH and the solution was subjected to reversed-phase HPLC with an elution system of a linear gradient of methanol in 10 mM Tris-HCl buffer (pH 6.0) and a detection system monitoring the absorbance of the effluent at 400 nm. The present method was shown to be utilized as a satisfactory method for the determination of γ-aminobutyric acid in foodstuffs.

• 2009-06-01

著者

• ISHIKAWA Akiko

  Department of Nutritional Science, Okayama Prefectural University

• OKA Hiroyuki

  Okayama Research Laboratory, Nihon-shokusei Co. Ltd.

• HIEMORI Miki

  Department of Nutritional Science, Okayama Prefectural University

• YAMASHITA Hiromi

  Department of Nutritional Science, Okayama Prefectural University

• KIMOTO Masumi

  Department of Nutritional Science, Okayama Prefectural University

• KAWASAKI Hitoshi

  Okayama Research Laboratory, Nihon-shokusei Co. Ltd.

• TSUJI Hideaki

  Department of Nutritional Science, Okayama Prefectural University

• Kimoto Masumi

  Department Of Nutritional Science Faculty Of Health And Welfare Okayama Prefectural University

• Oka Hiroyuki

  Okayama Research Laboratory Nihon-shokusei Co. Ltd.

• Tsuji Hiroaki

  Nisshin Oil Mills Ltd.

• Ishikawa Akiko

  Department Of Nutritional Science Okayama Prefectural University

• Ishikawa Akiko

  Department Of Community Health Tokai University School Of Medicine

• Tsuji H

  Dep. Of Nutritional Sci. Fac. Of Health And Welfare Sci. Okayama Prefectural Univ.

• Tsuji Hideaki

  Graduate School Of Health And Welfare Science Okayama Prefectural University

• Tsuji Hideaki

  Department Of Chemistry Faculty Of Education Gifu University

• Kimoto Masumi

  Department Of Nutritional Science Okayama Prefectural University

• Tsuji Hideaki

  Department Of Nutritional Science Okayama Prefectural University

• Yamasita Hiromi

  Dep. Of Nutritional Sci. Fac. Of Health And Welfare Sci. Okayama Prefectural Univ.

• Kawasaki Hitoshi

  Okayama Research Laboratory Nihon-shokusei Co. Ltd.

• Yamashita H

  Dep. Of Nutritional Sci. Fac. Of Health And Welfare Sci. Okayama Prefectural Univ.

• Yamashita Hiromi

  Department Of Applied Chemistry College Of Engineering Osaka Prefecture University

• Horiguchi M

  Department Of Nutritional Science Faculty Of Health And Welfare Science Okayama Prefectural Universi

• Kimoto M

  Department Of Nutritional Science Faculty Of Health And Welfare Science Okayama Prefectural Universi

• Kimoto Masumi

  Department of Immunology and Host Defenses, Ehime University Graduate School of Medicine

• Hiemori Miki

  Department of Nutritional Science, Faculty of Health and Welfare Science, Okayama Prefectural University

• HIEMORI Miki

  Department of Nutrition, School of Medicine, The University of Tokushima

関連論文

• Development of a Method for the Determination of γ-Aminobutyric Acid in Foodstuffs
• DYNAMIC CHANGE OF CELLS EXPRESSING MMP-2 mRNA AND MT1-MMP mRNA IN THE RECOVERY FROM RAT LIVER FIBROSIS
• Effect of Current Density and Organic Additives on the Texture and Hardness of Ni Electrodeposited from Sulfamate and Watt's Solutions
• Isolation and molecular cloning of a major wheat allergen, Tri a Bd 27K
• Purification and Partial Characterization of Acetyl-CoA Synthetase in Rat Liver Mitochondria
• Effect of Dietary Seal and Fish Oils on Lipid Metabolism in Hamsters
• Effect of Dietary Seal and Fish Oils in Triacylglycerol Metabolism in Rats
• Lymphatic Absorption of Seal and Fish Oils and Their Effect on Lipid Metabolism and Eicosanoid Production in Rats
• Thermostabilization of Ovotransferrin by Anions for Pasteurization of Liquid Egg White
• Structural Characteristics of the Disulfide-reduced Ovotransferrin N-Lobe Analyzed by Protein Fragmentation
• Structural and Immunochemical Homologies between Foxtail Millet Glutelin 60 kDa and Starch Granule-bound Starch Synthase Proteins from Rice, Barley, Corn and Wheat Grains
• Efficient Extraction and Some Properties of Storage Proteins (Prolamin and Glutelin) in Ancient Rice Cultivars
• Antigenic Homology between Buckwheat and Wheat Prolamins
• Immunochemical Crossreactivity between Globulins from Buckwheat and Indigo Seeds
• Isolation and Molecular Cloning of a Major Wheat Allergen, Tri a Bd 27K
• Preparation and Epitope Mapping of a Monoclonal Antibody against Tri a Bd 27K, a Major Wheat Allergen
• Characterization of Inhibitors of Postprandial Hyperglycemia from the Leaves of Nerium indicum
• Effect of a Hypolipidemic Drug, Di (2-ethylhexyl) phthalate, on mRNA-Expression Associated Fatty Acid and Acetate Metabolism in Rat Tissues
• Identification of a Wheat Allergen, Tri a Bd 36K, as a Peroxidase(Food & Nutrition Science)
• Sandwich Enzyme-linked Immunosorbent Assay System for Micro-detection of the Wheat Allergen, Tri a Bd 17 K(Food & Nutrition Science)
• Immunochemical Characterization of Ovomucoid from Japanese Quail Egg White Using Monoclonal Antibodies
• Quantitative Analysis of Gly m Bd 28K in Soybean Products by a Sandwich Enzyme-Linked Immunosorbent Assay
• Substantially Complete Removal of Three Major Allergenic Soybean Proteins (Gly m Bd 30K, Gly m Bd 28K, and the α-Subunit of Conglycinin) from Soy Protein by Using a Mutant Soybean, Tohoku 124
• Purification and Characterization of Soybean Allergen Gly m Bd 28K
• Micro-assay Method for Evaluating the Allergenicity of the Major Soybean Allergen, Gly m Bd 30K, with Mouse Antiserum and RBL-2H3 Cells
• Epitope Analysis of Soybean Major Allergen Gly m Bd 30K Recognized by the Mouse Monoclonal Antibody Using Overlapping Peptides
• Identification of the Glycosylation Site of a Major Soybean Allergen, Gly m Bd 30K
• Reduction of the Allergenicity of Soybean by Treatment with Proteases
• Micro-assay to Measure the Allergenicity of a Kunitz-type Soybean Trypsin Inhibitor toward Balb/c Mice by Using RBL-2H3 Cells
• α-Subunit of β-Conglycinin, an Allergenic Protein Recognized by IgE Antibodies of Soybean-sensitive Patients with Atopic Dermatitis
• Detection of N^G, N^G-Dimethylarginine Dimethylaminohydrolase in Human Tissues Using a Monoclonal Antibody^1
• Measurement of Gly m Bd 30K, a Major Soybean Allergen, in Soybean Products by a Sandwich Enzyme-linked Immunosorbent Assay
• Identification of the Soybean Allergenic Protein, Gly m Bd 30K, with the Soybean Seed 34-kDa Oil-body-associated Protein
• Identification of a Soybean Protein Producing IgE Antibodies in BALB/c Mice
• Analysis of Isoflavone Content in Tempeh, a Fermented Soybean, and Preparation of a New Isoflavone-Enriched Tempeh(BREWING AND FOOD TECHNOLOGY)
• An Isozyme of Earthworm Serine Proteases Acts on Hydrolysis of Triacylglycerol
• Purification and Characterization of α-Keto Amide Reductase from Saccharomyces cerevisiae
• Comparison of Acylated Plant Pigments : Light-resistance and Radical-scavenging Ability(Biochemistry & Molecular Biology)
• Further Stabilization of Earthworm Serine Protease by Chemical Modification and Immobilization(Biochemistry & Molecular Biology)
• Photoluminescence Properties of Mo-MCM-41 Mesoporous Molecular Sieves and Their Photocatalytic Reactivity for the Decomposition of NOx
• Photocatalytic Reduction of CO_2 with H_2O on Ti-MCM-41 and Ti-MCM-48 Mesoporous Zeolites at 328 K
• Photoluminescence Property of Titanium Silicalite-2 Catalyst and Its Photocatalytic Reactivity for the Direct Decomposition of NO at 295 K
• Thermal Rearrangement of N-Alkyl-, and N-Aryl-(2,2-dihalo-1-phenylcyclopropyl)methyleneamines to 1-Alkyl-,and 1-Aryl-2 (or 3)-halo-4-phenylpyrroles
• Investigation of Multiple Forms of Tri a Bd 27K, a Major Wheat Allergen, by Immunoblotting Analysis
• ビール中の新しい18-kDaのIgE結合蛋白質
• Attenuated Listeria infection activates natural killer cell cytotoxicity to regress melanoma growth in vivo
• Differential Effect of Listeria monocytogenes Infection on Cytokine Production and Cytotoxicity of CD8 T Cells
• A two-step model of T cell subset commitment : antigen-independent commitment of T cells before encountering nominal antigen during pathogenic infections
• The Characteristics of the Fluorescence Spectra of Rhodamine B Molecules Doped in Si-Ti Binary Oxide System by the Sol-Gel Method
• Molecular Cloning and Allergenicity of Pen j 1, a Major Allergen of Kuruma Prawn, Penaeus japonicus
• Reduction of CO_2 with H_2O on TiO_2(100) TiO_2(110) Single Crystals under UV-irradiation
• Effect of α-Cyclodextrin on Thermostability of Glucoamylase(Biological Chemistry)
• Photoluminescence Properties of the Vanadium Silicalite-1 Catalyst and Its Photocatalytic Reactivity for the Reduction of NO with Propane
• The Photocatalytic Decomposition of Nitric Oxide on the Silver(I) Ion-exchanged ZSM-5 Catalyst
• Effects of Acetate on Lipid Metabolism in Muscles and Adipose Tissues of Type 2 Diabetic Otsuka Long-Evans Tokushima Fatty (OLETF) Rats
• Improvement of Obesity and Glucose Tolerance by Acetate in Type 2 Diabetic Otsuka Long-Evans Tokushima Fatty (OLETF) Rats
• Limited Proteolysis of Disulfide-reduced Ovalbumin by Subtilisin
• A Case of Impacted Supernumerary Fourth Molar in the Bilateral Mandibular Ramus
• Modulation of the Immune System by Listeria monocytogenes-Mediated Gene Transfer into Mammalian Cells
• The Photocatalytic Isomerization of 2-Butenes on ZrO_2 Catalyst with Low Coordinated Surface Sites
• Preparation of TiO_2 Photocatalysts by Multi-gelation and Their Photocatalytic Reactivity for the Degradation of 2-Propanol
• Photocatalytic Degradation of 2-Propanol Diluted in Water with TiO_2 Photocatalyst Loaded on Si_3N_4
• Photophysicochemical Properties of Chlorophyll-a Adsorbed on Mg-containing Mesoporous Silica
• Preparation of Hypoallergenic Soybean Protein with Processing Functionality by Selective Enzymatic Hydrolysis
• Femtosecond Diffuse Reflectance Spectroscopy on Some Standard TiO_2 Powder Catalysts
• 希土類総説(45)Thermodynamic properties of alloys and compounds containing rare earth elements at high temperatures (13)
• Chronic nonsteroidal anti-inflammatory drug (NSAID) use suppresses multiple CpG islands hyper methylation (CIHM) of tumor suppressor genes in the human gastric mucosa
• Investigation of Multiple Forms of Tri a Bd 27K, a Major Wheat Allergen, by Immunoblotting Analysis
• Tumor-Suppressive Lipoxygenases Inhibit the Expression of c-myc mRNA Coding Region Determinant-Binding Protein/Insulin-Like Growth Factor II mRNA-Binding Protein 1 in Human Prostate Carcinoma PC-3 Cells
• Effects of Acetate on Lipid Metabolism in Muscles and Adipose Tissues of Type 2 Diabetic Otsuka Long-Evans Tokushima Fatty (OLETF) Rats
• Preparation and Epitope Mapping of a Monoclonal Antibody against Tri a Bd 27K, a Maior Wheat Allergen
• Molecular Cloning and Allergenicity of Pen j 1, a Major Allergen of Kuruma Prawn, Penaeus japonicus
• Isolation and Molecular Cloning of a Major Wheat Allergen, Tri a Bd 27K
• ビール中の新しい18-kDaのIgE結合蛋白質
• Improvement of Obesity and Glucose Tolerance by Acetate in Type 2 Diabetic Otsuka Long-Evans Tokushima Fatty (OLETF) Rats
• Effect of a Hypolipidemic Drug, Di (2-ethylhexyl) phthalate, on mRNA-Expression Associated Fatty Acid and Acetate Metabolism in Rat Tissues
• Fluorescence Properties of BDD Molecules Doped in Si-Ti Binary Oxide Systems by the Sol-Gel Method
• Photocatalytic Reduction of CO_2 with H_2O on Titanium Oxides Prepared within the FSM-16 Mesoporous Zeolite
• Gastrointestinal Digestion and Absorption of Pen j 1, a Major Allergen from Kuruma Prawn, Penaeus japonicus
• Quantitative Analysis of Gly m Bd 28K in Soybean Products by a Sandwich Enzyme-Linked Immunosorbent Assay
• Reduction of the Allergenicity of Soybean by Treatment with Proteases
• Effect of Organic Additives on the Hardness of Ni Electrodeposited from Sulfamate and Watt's Solutions
• Purification and Partial Characterization of Acetyl-CoA Synthetase in Rat Liver Mitochondria
• Lipocalin-Type Prostaglandin D Synthase and Egg White Cystatin React with IgE Antibodies from Children with Egg Allergy
• Analysis and Functionality of Major Polyphenolic Components of Polygonum cuspidatum (Itadori)
• Development of an Enzyme-Linked Immunosorbent Assay System for the Determination of Asymmetric Dimethylarginine Using a Specific Monoclonal Antibody
• Asymmetric Dimethylarginine, an Endogenous NOS Inhibitor, Is Actively Metabolized in Rat Erythrocytes
• Asymmetric Dimethylarginine, an Endogenous NOS Inhibitor, Is Actively Metabolized in Rat Erythrocytes
• Identification of Two Metabolites of Radioactive Shikimic Acid in Agaricus bisporus
• Reduction of the Soybean Allergenicity by the Fermentation with Bacillus natto.
• Fate of a Major Soybean Allergen, Gly m Bd 30K, in Rice-, Barley- and Soybean-Koji Miso (Fermented Soybean Paste) during Fermentation.
• Electroejaculation and Semen Characteristics of the Captive Hokkaido Brown Bear. (Ursus arctos yesoensis)
• Preparation and Application of Monoclonal Antibodies for a Sandwich Enzyme-Linked Immunosorbent Assay of the Major Soybean Allergen, Gly m Bd 30K.
• Investigation of the IgE-Binding Proteins in Soybeans by Immunoblotting with the Sera of the Soybean-Sensitive Patients with Atopic Dermatitis.
• Dioscorea japonica extract down-regulates prostaglandin E2 synthetic pathway and induces apoptosis in lung cancer cells

もっと見る 閉じる

スポンサーリンク