Purification and Partial Characterization of Acetyl-CoA Synthetase in Rat Liver Mitochondria

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概要

• 論文の詳細を見る

• Acetyl-CoA synthetase (AceCS), which catalyzes the activation of acetate to produce acetyl-CoA, was found to have a much greater Km value for acetate in liver mitochondria than that in the heart mitochondria of rats, indicating that two different types of AceCS are located in the liver and heart mitochodria. Recently, Fujino et al. reported that mouse heart mitochondrial AceCS, designated AceCS2, was expressed in a wide range of tissues, however, it was apparently absent from the liver. In this study, liver mitochondrial AceCS activity, but not heart AceCS2, was greatly induced in di(2-ethylhexyl)phthalate (DEHP)-treated rats. We purified and characterized the rat liver mitochondrial AceCS. The molecular mass of the enzyme estimated by SDS-PAGE was -58 kDa, which was quite different from that of the heart mitochondrial enzyme, AceCS2. The calculated Km value for the acetate of the partially purified liver enzyme was much greater, being about 100 times that of heart enzyme, AceCS2.

• 財団法人 学会誌刊行センターの論文
• 2002-10-01

著者

• HIEMORI Miki

  Department of Nutritional Science, Okayama Prefectural University

• Kimoto Masumi

  Department Of Nutritional Science Faculty Of Health And Welfare Okayama Prefectural University

• Tsuji Hiroaki

  Nisshin Oil Mills Ltd.

• Tsuji H

  Dep. Of Nutritional Sci. Fac. Of Health And Welfare Sci. Okayama Prefectural Univ.

• Tsuji Hideaki

  Department Of Chemistry Faculty Of Education Gifu University

• Nakamura Tomomi

  Department Of Nutritional Science Faculty Of Health And Welfare Science Okayama Prefectural Universi

• Tsuji Hideaki

  Department Of Nutritional Science Okayama Prefectural University

• Yamasita Hiromi

  Dep. Of Nutritional Sci. Fac. Of Health And Welfare Sci. Okayama Prefectural Univ.

• Fukuura Akemi

  Department Of Nutritional Science Faculty Of Health And Welfare Science Okayama Prefectural Universi

• Kaneyuki Takao

  Department Of Nutritional Science Faculty Of Health And Welfare Science Okayama Prefectural Universi

• Yamashita Hiromi

  Department Of Applied Chemistry College Of Engineering Osaka Prefecture University

• Horiguchi M

  Department Of Nutritional Science Faculty Of Health And Welfare Science Okayama Prefectural Universi

• Kimoto M

  Department Of Nutritional Science Faculty Of Health And Welfare Science Okayama Prefectural Universi

• Nakamura T

  Department Of Nutritional Science Faculty Of Health And Welfare Science Okayama Prefectural Universi

• Kaneyuki T

  Department Of Nutritional Science Faculty Of Health And Welfare Science Okayama Prefectural Universi

• Fukuura Akemi

  Department Of Nutritional Science Faculty Of Health And Welfare Science Okayama Prefectural Universi

• Kimoto Masumi

  Department of Immunology and Host Defenses, Ehime University Graduate School of Medicine

• Hiemori Miki

  Department of Nutritional Science, Faculty of Health and Welfare Science, Okayama Prefectural University

• HIEMORI Miki

  Department of Nutrition, School of Medicine, The University of Tokushima

• Kaneyuki Takao

  Department of Neurology, Okayama University Medical School

• Nakamura Tomomi

  Department of Internal Medicine, Osaka City General Hospital, Japan

• Nakamura Tomomi

  Department of Biochemistry, School of Medicine, Keio University

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