Sandwich Enzyme-linked Immunosorbent Assay System for Micro-detection of the Wheat Allergen, Tri a Bd 17 K(Food & Nutrition Science)

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概要

• 論文の詳細を見る

• Seven monoclonal antibodies (mAbs) against the wheat allergen, Tri a Bd 17 K, were prepared to obtain mAbs suitable for a sandwich enzyme-linked immunosorbent assay (sandwich ELISA) for determination of the allergen. Two of the mAbs strongly immunoblotted the allergen purified from wheat flour. However, only one (1G11) of them was found to be suitable for sandwich ELISA. Epitope mapping against mAb-lGll on the allergen showed that the mAb recognized the peptide containing Lys-38 and Gln-39 of the allergen. We developed a sandwich ELISA method consisting of Aleuria aurantia lectin for fixing the allergen and 1G11 as the first antibody that enabled 4-4,000 ng/well of the allergen to be determined.

• 社団法人日本農芸化学会の論文
• 2001-12-23

著者

• HIEMORI Miki

  Department of Nutritional Science, Okayama Prefectural University

• YAMASHITA Hiromi

  Department of Nutritional Science, Okayama Prefectural University

• TSUJI Hideaki

  Department of Nutritional Science, Okayama Prefectural University

• Kimoto Masumi

  Department Of Nutritional Science Faculty Of Health And Welfare Okayama Prefectural University

• Tsuji Hiroaki

  Nisshin Oil Mills Ltd.

• Tsuji H

  Dep. Of Nutritional Sci. Fac. Of Health And Welfare Sci. Okayama Prefectural Univ.

• Tsuji Hideaki

  Graduate School Of Health And Welfare Science Okayama Prefectural University

• Tsuji Hideaki

  Department Of Chemistry Faculty Of Education Gifu University

• Suzuki Kazuhiko

  Department of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The Universit

• Kimoto Masumi

  Department Of Nutritional Science Okayama Prefectural University

• OKITA Misako

  Department of Nutritional Science, Faculty of Health and Welfare Science, Okayama Prefectural Univer

• Tsuji Hideaki

  Department Of Nutritional Science Okayama Prefectural University

• Okita M

  Organic Synthesis Research Laboratory Sumitomo Chemistry Co. Ltd.

• Okita Misako

  Department Of Food Science And Nutrition Nara Women's University

• Yamasita Hiromi

  Dep. Of Nutritional Sci. Fac. Of Health And Welfare Sci. Okayama Prefectural Univ.

• Yamashita H

  Dep. Of Nutritional Sci. Fac. Of Health And Welfare Sci. Okayama Prefectural Univ.

• Yamashita Hiromi

  Department Of Applied Chemistry College Of Engineering Osaka Prefecture University

• Horiguchi M

  Department Of Nutritional Science Faculty Of Health And Welfare Science Okayama Prefectural Universi

• Kimoto M

  Department Of Nutritional Science Faculty Of Health And Welfare Science Okayama Prefectural Universi

• Suzuki Kazuhiko

  Department Of Electrical Engineering Hokkaido Institute Of Technology

• Suzuki Kazuhiko

  Department Of Nutritional Science Faculty Of Health And Welfare Science Okayama Prefectural Universi

• Kimoto Masumi

  Department of Immunology and Host Defenses, Ehime University Graduate School of Medicine

• Hiemori Miki

  Department of Nutritional Science, Faculty of Health and Welfare Science, Okayama Prefectural University

• HIEMORI Miki

  Department of Nutrition, School of Medicine, The University of Tokushima

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