Characterization of Inhibitors of Postprandial Hyperglycemia from the Leaves of Nerium indicum

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概要

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• Nerium indicum is an India-Pakistan-originated shrub belonging to the oleander family. The ingestion of leaves of N. indicum before a meal is known to effect the lowering of postprandial glucose levels in Type II diabetic patients and this plant is now used as a folk remedy for Type II diabetes in some regions of Pakistan. In the present study, the hot-water extract of N. indicum leaves was found to reduce the postprandial rise in the blood glucose when maltose or sucrose was loaded in rats. It was also found that the extract strongly inhibited α-glucosidase, suggesting that the suppression of the postprandial rise in the blood glucose is due to the occurrence of some inhibitors of α-glucosidase in the leaves. We, therefore, tried to isolate the active principles from the leaf extract, using α-glucosidase-inhibitory activity as the index. Employing Sephadex G-15, silica gel and reversed-phase HPLC, we isolated two active compounds. The UV, mass and NMR spectrometric analyses established that the chemical structures of these compounds are 3-O-caffeoylquinic acid (chlorogenic acid) and its structural isomer, 5-O-caffeoylquinic acid. Both compounds were shown to inhibit α-glucosidases in a non-competitive manner. The authentic chlorogenic acid was found to suppress the postprandial rise in the blood glucose in rats and also inhibited the absorption of the glucose moiety from maltose and glucose in the everted gut sac system prepared from rat intestine. These results demonstrate that chlorogenic acid is one of the major anti-hyper-glycemic principles present in the leaves of N. indicum. Furthermore, among polyphenol compounds tested, quercetin and catechins were shown to have strong inhibitory activity against α-glucosidase.

• 日本ビタミン学会の論文

著者

• ISHIKAWA Akiko

  Department of Nutritional Science, Okayama Prefectural University

• HIEMORI Miki

  Department of Nutritional Science, Okayama Prefectural University

• YAMASHITA Hiromi

  Department of Nutritional Science, Okayama Prefectural University

• TSUJI Hideaki

  Department of Nutritional Science, Okayama Prefectural University

• Natori Yasuo

  Institute Of Health And Nutrition Nagoya University Of Arts And Sciences

• Inagaki Eiko

  Institute Of Health And Nutrition Nagoya University Of Arts And Sciences

• Kimoto Masumi

  Department Of Nutritional Science Faculty Of Health And Welfare Okayama Prefectural University

• Memon Allah

  Institute Of Biochemistry University Of Sindh

• Tsuji Hiroaki

  Nisshin Oil Mills Ltd.

• Ishikawa Akiko

  Department Of Nutritional Science Okayama Prefectural University

• Ishikawa Akiko

  Department Of Community Health Tokai University School Of Medicine

• Tsuji H

  Dep. Of Nutritional Sci. Fac. Of Health And Welfare Sci. Okayama Prefectural Univ.

• Tsuji Hideaki

  Graduate School Of Health And Welfare Science Okayama Prefectural University

• Tsuji Hideaki

  Department Of Chemistry Faculty Of Education Gifu University

• Kimoto Masumi

  Department Of Nutritional Science Okayama Prefectural University

• OKAMOTO Masahiko

  Organic Synthesis Research Laboratory, Sumitomo Chemistry Co. Ltd.

• MOHAMMADI Alli

  Department of Radio-Diagnosis and Imaging, Isra University

• Tsuji Hideaki

  Department Of Nutritional Science Okayama Prefectural University

• Okita M

  Organic Synthesis Research Laboratory Sumitomo Chemistry Co. Ltd.

• Yamasita Hiromi

  Dep. Of Nutritional Sci. Fac. Of Health And Welfare Sci. Okayama Prefectural Univ.

• Mohammadi Alli

  Department Of Radio-diagnosis And Imaging Isra University

• Yamashita H

  Dep. Of Nutritional Sci. Fac. Of Health And Welfare Sci. Okayama Prefectural Univ.

• Yamashita Hiromi

  Department Of Applied Chemistry College Of Engineering Osaka Prefecture University

• Horiguchi M

  Department Of Nutritional Science Faculty Of Health And Welfare Science Okayama Prefectural Universi

• Kimoto M

  Department Of Nutritional Science Faculty Of Health And Welfare Science Okayama Prefectural Universi

• Kimoto Masumi

  Department of Immunology and Host Defenses, Ehime University Graduate School of Medicine

• Hiemori Miki

  Department of Nutritional Science, Faculty of Health and Welfare Science, Okayama Prefectural University

• HIEMORI Miki

  Department of Nutrition, School of Medicine, The University of Tokushima

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