Most Clot-Bound Thrombin Activity is Inhibited by Plasma Antithrombin during Clot Aging, but a Very Small Fraction Survives
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概要
- 論文の詳細を見る
Inhibitory effects of thrombin inhibitors against clot-bound throumbin have been evaluated using clots prepared from human plasma as the source of clot-immobilized active enzyme, and the clot-bound thrombin has been reported to be protected from its inhibition by antithrombin. However, we found that the clot-bound thrombin was not intrinsically protected from inhibition by antithrombin, i.e., a large fraction of intially active clotbound thrombin mas inhibited by antithrombin present in human plasma time dependently, and only very small fractions (0.04-0.08%) of the thronbin retained their enzymatic activity after clot-aging. These results suggest that the extent of clot-aging determines the sensitivity of clot-bound thrombin to antithrombin, and that inhibitory effects of drugs against clot-bound thrombin in vitro must be interpreted with caution to estimate their effects in vivo.
- 公益社団法人日本薬学会の論文
- 2000-04-01
著者
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Hayashi Hideya
Pharmaceuticals & Biotechnology Laboratory Japan Energy Corporation
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KOMATSU Yasuhiko
Pharmaceuticals and Biotechnology Laboratory, Japan Energy Corporation
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Komatsu Yasuhiko
Medical Proteoscope Co. Ltd.
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Komatsu Yasuhiko
Pharmaceuticals & Biotechnology Laboratory Japan Energy Corporation
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