Mg^<2+>-ATPASE ACTIVITY DURING ENDOCYTOSIS OF CATIONIZED FERRITIN IN MOUSE MACROPHAGES : A DOUBLE LABELING STUDY

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To study the flow of membrane constituents during the course of endocytosis, cytochemically-demonstrable Mg^<2+>-adenosine triphosphatase (Mg^<2+>-ATPase) activity was chosen as a marker of the cell membrane and was detected during the course of the endocytosis of cationized ferritin (CF) by the double labeling technique. Mouse peritoneal macrophages were incubated with CF at 37℃ for 30-60 min, fixed, and then processed for the demonstration of Mg^<2+>-ATPase activity. Mg^<2+>-ATPase activity was found in CF-labeled vesicles and vacuoles. Mg^<2+>-ATPase activity, however, was not detected in multivesicular bodies, in lysosomes, or in the Golgi apparatus, although endocytosed CF was found in these organelles. These results suggest that Mg^<2+>-ATPase was co-internalized with CF and that its activity was lost during the translocation to lysosomes and the Golgi apparatus, presumably owing to inactivation and membrane recycling.
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