Effects of TRH or CB-154 on Functional Pituitary Adenoma Cells in Culture Revealed by Their Ultrastructural Study Using Cationized Ferritin
スポンサーリンク
概要
- 論文の詳細を見る
In order to clarify the mechanisms of action of CB-154 and TRH, ultrastructural studies using cationized ferritin as a ligand for anionic sites of membrane constituents of human adenoma cells were performed. Adenoma cells were cultured for a certain period with intermittent incubation with either CB-154 or TRH. The effects of these agents were ascertained by hormonal analysis of the culture media. We examined 7GH-producing adenomas, 4 PRL-producing ones, and 2 non-functioning ones. In functioning adenoma cells, hormone secretion was confirmed to be increased by TRH activation and decreased by CB-154 incubation. Hormonal examination of cultured media revealed autonomous secretion of PRL but not of GH in dispersed cell cultures of human pituitary adenomas. When activated by TRH, both GH and PRL adenoma cells contained abundant cationized ferritin (CF) particles in the cytoplasm in the form of densely packed small vacuoles, endoplasmic reticulum (ER), and Golgi cisternae. In CB-154 treated cells, internalization of CF was rarely observed, as if inhibited by the action of CB. Internalization of CF particles in the control study seemed to lie in degree between the TRH activated cells and the CB-154 treated ones. It was made clear in this study that TRH activated the recovery or recycling of surface membrane in functioning adenoma cells, while CB-154 seemed to suppress the internalization of anionic sites of the surface membrane.
- 日本脳神経外科学会の論文
- 1983-01-15
著者
-
HORI Tomokatsu
Division of Neurosurgery, Institute of Neurological Sciences
-
HIRANO Hiroshi
Department of Anatomy, Kyorin University School of Medicine
-
Hori Tomokatsu
Division Of Neurosurgery Institute Of Neurological Sciences Tottori University School Of Medicine
-
Numata Hideharu
Division Of Neurosurgery Institute Of Neurological Sciences Tottori University School Of Medicine
-
Saito Yoshikazu
Division Of Neurosurgery Institute Of Neurological Sciences Tottori University School Of Medicine
-
NISHIYAMA Fumiaki
Department of Anatomy, School of Health Sciences, Kyorin University
-
HOKAMA Yasuo
Division of Neurosurgery,Institute of Neurological Sciences,Tottori University School of Medicine
-
MURAOKA Kiyoaki
Division of Neurosurgery,Institute of Neurological Sciences,Tottori University School of Medicine
-
TAKAMI Masami
Division of Neurosurgery, Institute of Neurological Sciences, Tottori University School of Medicine
-
Hokama Yasuo
Division Of Neurosurgery Institute Of Neurological Sciences Tottori University School Of Medicine
-
Takami Masami
Division Of Neurosurgery Institute Of Neurological Sciences Tottori University School Of Medicine
-
Nishiyama Fumiaki
Department Of Anatomy Kyorin University School Of Medicine
-
Hori Tomokatsu
Division Of Neurosurgery Institute Of Neurological Sciences
-
Muraoka Kiyoaki
Division Of Neurosurgery Institute Of Neurological Sciences Tottori University School Of Medicine
-
Hirano Hiroshi
Department Of Anatomy Kansai Medical School
-
Hirano Hiroshi
Department Of 2nd Anatomy Kyorin University School Of Medicine
-
HORI Tomokatsu
Division of Neurosurgery,Institute of Neurological Sciences,Tottori University School of Medicine
関連論文
- Co-localization of Human Growth Hormone and Human Prolactin in Hormonally Pure Human Growth Hormone-producing Pituitary Adenomas
- Magnetic Resonance Imaging in Kainic Acid-induced Limbic Seizure Status in Cats
- Salicylic Acid Carboxyl Methyltransferase Induced in Hairy Root Cultures of Atropa belladonna after Treatment with Exogeneously Added Salicylic Acid
- Ameliorated Outcome of Subarachnoid Hemorrhage Patients Treated with Reduced Form Glutathione
- MS2-4 Morphometrical evaluation of androgen action in testis and prostate
- Desmoplastic malignant mesothelioma : two cases and a literature review
- Immunohistochemical Localization of β-1, 4-Galactosyltransferase in Human Pancreatic Tissues
- Chordoma in the Sella Turcica
- Pulmonary pleomorphic carcinoma producing hCG
- Poorly Differentiated Adenocarcinoma with Signet-Ring Cell Carcinoma in a Hyperplastic Polyp of the Stomach : Report of a Case
- Myosin Light Chain Phosphorylation is Correlated with Cold-Induced Changes in Platelet Shape
- Growth Inhibition of Helicobacter pylori by Monoclonal Antibody to Heat-Shock Protein 60
- Renal adenomatosis associated with carcinoma of the lower urinary tract : A case report with immunohistochemical study
- Leiomyosarcoma arising from soft tissue tumor of the mediastinum
- Malignant mesothelioma of the pericardium : Case reports and immunohistochemical studies including Ki-67 expression
- Synovial sarcoma arising from the pleura : a case report with ultrastructural and immunohistological studies
- Changes in AlPase Activity of Rabbit Articular Cartilages Associated with Aging and Joint Contracture
- Malignant mesothelioma of the peritoneum : case reports and immunohistochemical findings including Ki-67 expression
- Responses of Transformed Roots of Atropa Belladonna to Treatment with Salicylic Acid and other Phenolic Compounds
- Responses of Transformed Root Culture of Atropa belladonna to Salicylic Acid Stress
- Detection of Disialoganglioside in Rat Cerebellar Cortex by Light and Electron Microscopy
- P3-31 Distribution of the Long Form of Type XII Collagen during Chicken Corneal Formation
- Binding of Griffonia simplicifolia I-B_4 Lectin in the Substantia Gelatinosa of the Rat Spinal Cord
- P-47 Binding of Griffonia simplicifolia I-B_4 lectin in the substantia gelatinosa of rat spinal cord.
- Changes in HRP penetration into the articular cartilage of the experimentally contracted joint.
- The demonstration of the intracytoplasmic lectin binding sites in the granulocytes by means of ultracryotomy.
- Subcellular Localization of Galactosyltransferase Associated with Tumors in Endometrial and Ovarian Cancer Cells
- GLUT1 Glucose Transporter in the Lactating Mammary Gland in the Rat
- O-GlcNAc modification of nucleocytoplasmic proteins and diabetes
- Elevated Post-Translational Modification of Proteins by O-Linked N-Acetylglucosamine in Various Tissues of Diabetic Goto-Kakizaki Rats Accompanied by Diabetic Complications
- P-36-A Diabetic keratopathy and O-GlcNAc transferase in corneas of diabetic Goto-Kakizaki rat(THE JOINT MEETING OF THE 44TH ANNUAL MEETING OF THE JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY AND THE 35TH ANNUAL MEETING OF THE CLINICAL ELECTRON MICROS
- Phenotypic Alteration of Carbohydrate Antigens in Gynecological Malignancy
- Lectin-binding Patterns of the Normal and Malignant Endometrium.
- Importance of Carbohydrates and Platelets in the Early Events of Cancer Metastasis (Recent Progresses in Carbohydrate Histochemistry)
- Changes in Lectin-binding Patterns of the Rat Gastric Mucosa Induced by Stress Ulcer
- Changes in Lectin Binding Patterns of Human Pulmonary Tissues in Association with Congenital Heart Diseases
- Thymic Carcinoma with a Large Cystic Lesion
- Localized pleural malignant mesothelioma
- Prognostic Significance Of Persistent Mediastinal Metastasis Following Induction Therapy In Large (>or=2cm) N2 or N3 Non-Small Cell Lung Cancer
- B-18 Histochemical Detection of Various Gangliosides in Rat Cerebellar Cortex
- Distribution of glycoconjugates in the colon carcinoma cells during the process of liver metastasis
- DBA-staining Pattern of Stimulated Human Gastric Parietal Cells : A Microspectrophotometric Study
- Light and Electron Microscopic Study of Lectin Binding Sites in Human Esophageal Epithelia
- Anaplastic large-cell lymphoma (Ki-1 lymphoma) : ultrastructural and immunohistochemical studies
- Case of primary malignant hemangiopericytoma of the heart expressing basement membrane-degradable enzymes
- Expression of Hex homeobox gene during skin development : Increase in epidermal cell proliferation by transfecting the Hex to the dermis
- Expression of matrix metalloproteinases, tissue inhibitors of metalloproteinase, collagens, and Ki67 antigen in pleural malignant mesothelioma : an immunohistochemical and electron microscopic study
- Pulmonary dirofilariasis : clinicopathological study
- Spiral artery of placenta : development and pathology-immunohistochemical, microscopical, and electron-microscopic study
- Increased exocytosis of secretory granules in contrast to reduced serum hormone levels in pituitary adenomas of humans and rats treated with dopamine agonist
- P-20 Immunolocalization of TrkB, a high-affinity neurotrophin receptor, in human olfactory mucosae
- AN ELECTRON MICROSCOPIC STUDY ON THE HORSERADISH PEROXIDASE-LABELED NEURON IN THE EPILEPTIC FOCUS
- P-57 Immunohistochemical study on cellular co-localization of estrogen and progesterone receptors, HSP90, and pS2 in breast cancer tissues
- Effects of Bromocriptine and Radiation on the Size and Activity of Estrogen-induced Rat Prolactinoms
- Effects of TRH or CB-154 on Functional Pituitary Adenoma Cells in Culture Revealed by Their Ultrastructural Study Using Cationized Ferritin
- USE OF FLUORESCEIN-PHALLOIDIN AND DAPI AS A COUNTERSTAIN FOR IMMUNOFLUORESCENCE MICROSCOPIC STUDIES WITH SEMITHIN FROZEN SECTIONS
- Identification and Properties of the Glucose Transporter of Human Erythrocytes(MEMBRANE PROTEINS AND TRANSPORT-II)
- Intrinsic Ureteric Involvement by Endometriosis : A Case Report
- Basic problems of digital image processing with personal computers
- P-58 Changes in expression of androgen receptor localized in spermatogonia in the cycle of the seminiferous epithelium: immunohistochemical, semi-quantitative study using the whole-mount method
- P-41-B Laser microscopic observations on the distribution of BDNFmRNA in the rat olfactory epithelium(THE JOINT MEETING OF THE 44TH ANNUAL MEETING OF THE JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY AND THE 35TH ANNUAL MEETING OF THE CLINICAL ELECTRO
- A Case Report : Rare Case of Primary Transitional Cell Carcinoma of the Fallopian Tube
- Immunohistochemical analyses of E-cadherin, β-catenin, CD44s, and CD44v6 expressions, and Ki-67 labeling index in intraductal papillary mucinous neoplasms of the pancreas and associated invasive carcinomas
- Immunohistochemical Localization of β-1, 4-Galactosyltransferase in Human Colonic Tissues
- SUGAR RESIDUES ON THE CELL SURFACE OF THE DISSOCIATED MOUSE KIDNEY TUBULE CELLS BY MEANS OF FERRITIN-LABELED LECTINS
- Comparison and Joint Use of the Peroxidase- and Ferritin-Labeling Methods on the Cell Surface Membranes (Symposium on the Enzyme-labeled Anitibody Method)
- Distribution of O-GlcNAc Transferase in the Rat Pancreas
- P-48 Glucose-induced Changes in O-GlcNAc Transferase Expression and Localization in Rat Aortic Smooth Muscle Cell
- Lectin Histochemical Study of Squamous Carcinoma in the Head and Neck Region
- Mg^-ATPASE ACTIVITY DURING ENDOCYTOSIS OF CATIONIZED FERRITIN IN MOUSE MACROPHAGES : A DOUBLE LABELING STUDY
- THE ENDOCYTOSIS AND THE INTRACELLULAR FATE OF CATIONIZED FERRITIN IN MONKEY MACROPHAGES
- Distribution of the Negative Charge on the Dissociated Thyroid Follicle Cell Surface
- Experimental Comparison of Flat-panel Detector Performance for Direct and Indirect Systems
- Stimulation by Urocortin of Growth Hormone (GH) Secretion in GH-Producing Human Pituitary Adenoma Cells
- O-GlcNAc Transferase in Pancreas of Rats : Elevated expression of O-GlcNAc Transferase with Streptozotocin-induced Diabetes
- Lectin Binding Pattern in Extramammary Paget's Disease
- Lectin Histochemistry and Cytochemistry using Ultrathin Frozen Sections
- Undifferentiated carcinoma with osteoclast-like giant cells arising in a mucinous cystic neoplasm of the pancreas
- Alterations of Con A Label on the Tumor Surface and Non-tumorous Mucosa of Human Urinary Bladder
- A case of mixed ductal-endocrine carcinoma of the pancreas
- Histochemical Detection of Sugar Residues by Joint Use of Specific Antibodies and Lectins
- 4. Ultrastructural Localization of the Activity of Phosphatases in the Normal Heart with Particular Reference to the Intramitochondrial Phoshpatases (Electron Microscopic Histo- and Cytochemistry,Recent Progress in Phosphatase Cytochemistry)
- The Practical Side of Image Processing with the Use of a Personal Computer : Automation of the Analysing Processes Employing NIH Image and Adobe Photoshop^ with Power Macintosh Computers (The Practical Side of Image Processing Using Pe
- Light and Electron Microscopic Studies on the Acetylcholinesterase (AChE) activity in Auerbach's Plexus of the Developing Rat Colon
- ATTEMPTS TO STAIN THE INTRACELLULAR ANTIGENS BY MEANS OF IMMUNO-FERRITIN METHOD (SYMPOSIUM ON THE RECENT ADVANCES IN THE ELECTRON MICROSCOPIC CYTOCHEMISTRY)
- Changes in Lectin Binding Patterns in Rat Testis and Epididymis in Association with Experimentally-Induced Cryptorchidism
- Expression of both hepatocellular carcinoma and cholangiocarcinoma phenotypes in hepatocellular carcinoma and cholangiocarcinoma components in combined hepatocellular and cholangiocarcinoma
- Localized pleural malignant mesothelioma
- Extra-gastrointestinal stromal tumor of the pelvic cavity : case report
- ULTRACYTOCHEMICAL DEMONSTRATION OF INTRACELLULAR AMINO GROUPS BY THE HYDROQUINONE-FERRICYANIDE SYSTEM
- Binding of cationized ferritin on the cytoplasmic surface of the axolemma and its effect on the membrane excitation.
- Okadaic Acid Induces Marked Shape Change of Human Platelets.
- Pulmonary tumor thrombotic microangiopathy showing aggressive course after transurethral resection of urinary bladder : an autopsy case report
- Concanavalin A (ConA) receptors in mouse macrophages demonstrated in thick sections with a 1,000kV transmission electron microscope.
- Glycoconjugates in Histochemistry and Cytochemistry