Expression of Sugar Transporters by In Vivo Electroporation and Particle Gun Methods in the Rat Liver : Localization to Specific Membrane Domains
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概要
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To see the cellular localization mechanism of membrane proteins in the hepatocyte in situ, we introduced cDNAs of facilitated-diffusion sugar transporters, GLUT1,GLUT3,GLUT4,and GLUT5 , and a Na^+ -dependent active sugar transporter SGLT1 into the rat liver by the in vivo electroporation method and the particle gun method, and the localization of their products was anaIyzed by immunofluorescence microscopy. SGLT1 was strictly restricted to the biIe canalicular (apical) domain, whereas GLUT1 was found in the sinusoidal (basolateral) membrane of hepatocytes. GLUT3 and GLUT5 were present along the whole aspects of the plasma membrane with a tendency for the bile canalicular membrane to be enriched with transporters as compared with the sinusoidal membrane. GLUT4 remained in the intracellular compartments. Simultaneous expression of two of the transporters confirmed these results. Compared with membrane localization of sugar transporters in MDCK cells, GLUT1,GLUT4 and SGLT1 exhibited a similar localization pattern.0n the other hand, localization of GLUT3 and GLUT5 was different from that in MDCK cells. These observations suggest that hepatocytes in situ may have a different localization mechanism for sugar transporters from that in MDCK ceIIs. In addition, the in vivo electroporation and the particIe gun methods seem to be useful tools for the introduction and analysis of foreign genes in the liver in situ.
- 日本組織細胞化学会の論文
著者
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KUWANO Hiroyuki
First Department of Surgery, Gunma University
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SUZUKI Takeshi
Laboratory of Soil Science, Faculty of Agriculture, Kobe University
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Suzuki T
Department Of Anatomy And Cell Biology Gunma University Graduate School Of Medicine
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Kuwano Hiroyuki
First Department Of Surgery Gunma University School Of Medicine
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Shinoda Yasuo
First Department of Surgery, Gunma University School Of Medicine
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Sugawara-Yokoo Minako
Laboratory of Molecular and Cellular Morphology, Department of Cell Biology, Institute for Molecular
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Nagamatsu Shinya
Department of Biochemistry, Kyorin University School of Medicine
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Takata Kuniaki
Laboratory of Molecular and Cellular Morphology, Department of Cell Biology, Institute for Molecular
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Kuwano Hiroyuki
First Department Of Surgery Gunma University
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Takata Kuniaki
Department Of Anatomy And Cell Biology Gunma University Graduate School Of Medicine
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Sugawara Minako
Laboratory Of Molecular And Cellular Morphology Department Of Cell Biology Institute For Molecular A
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Shinoda Yasuo
Department Of Anatomy And Cell Biology Gunma University School Of Medicine:first Department Of Surge
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Suzuki Takeshi
Laboratory Of Soil Science Faculty Of Agriculture Kobe University
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Nagamatsu Shinya
Department Of Biochemistry Kyorin University School Of Medicine
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Takata Kuniaki
Laboratory Of Molecular And Cellular Morphology Institute For Molecular And Cellular Regulation Gunm
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Takata Kuniaki
Laboratory Of Molecular And Cellular Morphology Gunma University
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Suzuki Takeshi
Laboratory Of Microbial Biochemistry Institute For Chemical Research Kyoto University
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Nagamatsu S
Kyorin Univ. School Of Medicine Tokyo Jpn
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Nagamatsu Shinya
Department Of Biochemistry Kyorin University School Of Medecine
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