Optimization of a Methylation Procedure to Obtain Chloroform-Soluble Humic Acids (Soil Chemistry and Mineralogy)
スポンサーリンク
概要
- 論文の詳細を見る
It will be important in future analyses of humic acids (HAs) by various chromatographic and spectrometric methods to obtain high yields of methylated HAs. A derivatization procedure for permethylation of HAs was evaluated in relation to the yield of the chloroform-soluble fraction. The procedure involved a 3 h incubation of HAs (50 mg) with methylsulfinyl carbanion (MSC) obtained by mixing petroleum-purified NaH with anhydrous dimethylsulfoxide (DMSO), followed by incubation with methyl iodide (CH_3I). Ten HAs isolated from different Japanese and American soils (three Andisols, four Inceptisols, one Histosol, one Alfisol, and one Ultisol) were used to optimize this procedure. Optimum derivatization was achieved within the first 6 h after the addition of MSC and CH_3I; further incubation (up to 24 h) did not result in significant changes in the yield of the chloroform-soluble fraction (60-100%). For most HAs, the maximal yields of chloroform-soluble fractions were obtained at concentrations of 10 mM for MSC and 13 mM for CH_3I.
- 社団法人日本土壌肥料学会の論文
著者
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YANAGI Yukiko
The Graduate School of Agricultural Science, Kobe University
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FUJITAKE Nobuhide
The Graduate School of Agricultural Science, Kobe University
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Yanagi Yukiko
The Graduate School Of Agricultural Science Kobe University
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Fujitake N
Laboratory Of Soil Science Faculty Of Agriculture Kobe University
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Fujitake Nobuhide
Laboratory Of Soil Science Faculty Of Agriculture Kobe University
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Ohta Hiroyuki
School Of Agriculture Ibaraki University
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Yanagi Yukiko
Laboratory Of Soil Science Faculty Of Agriculture Kobe University
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SUZUKI Takeshi
Laboratory of Soil Science, Faculty of Agriculture, Kobe University
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OTSUKA Hiroo
Laboratory of Soil Science, Faculty of Agriculture, Kobe University
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Otsuka H
Laboratory Of Soil Science Faculty Of Agriculture Kobe University
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Ohta H
Department Of Biological Sciences Graduate School Of Bioscience And Biotechnology Tokyo Institute Of
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Ohta Hiroyuki
Dept. Biol. Sci. Tokyo Inst. Technol.
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Ohta H
Okayama Univ. Dental School Okayama Jpn
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Ohta H
Ibaraki University College Of Agriculture
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Bollag Jean-Marc
ペンシルバニア州立大
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STRYNAR Mark
Laboratory of Soil Biochemistry, Center for Bioremediation and Detoxification, The Pennsylvania Stat
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MISHIMA Taketoshi
Laboratory of Soil Science, Faculty of Agriculture, Kobe University
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TSUKAMOTO Mayuko
Laboratory of Soil Science, Faculty of Agriculture, Kobe University
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DEC Jerzy
Laboratory of Soil Biochemistry, Center for Bioremediation and Detoxification, The Pennsylvania Stat
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BOLLAG Jean-Marc
Laboratory of Soil Biochemistry, Center for Bioremediation and Detoxification, The Pennsylvania Stat
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Fujitake N
Graduate School Of Agricultural Sci. Kobe Univ.
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Fujitake Nobuhide
The Graduate School Of Agricultural Science Kobe University
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Dec Jerzy
Laboratory Of Soil Biochemistry Center For Bioremediation And Detoxification The Pennsylvania State
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Strynar Mark
Laboratory Of Soil Biochemistry Center For Bioremediation And Detoxification The Pennsylvania State
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Bollag Jean-marc
Laboratory Of Soil Biochemistry Center For Bioremediation And Detoxification The Pennsylvania State
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Tsukamoto Mayuko
Laboratory Of Soil Science Faculty Of Agriculture Kobe University
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Fujitake N
Kobe Univ. Kobe Jpn
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Ohta H
Ibaraki Univ. Coll. Agriculture Ibaraki Jpn
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Suzuki Takeshi
Laboratory Of Soil Science Faculty Of Agriculture Kobe University
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Mishima Taketoshi
Laboratory Of Soil Science Faculty Of Agriculture Kobe University
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Suzuki Takeshi
Laboratory Of Microbial Biochemistry Institute For Chemical Research Kyoto University
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Fujitake Nobuhide
Laboratory Of Soil Science Department Of Agro-bioscience Graduate School Of Agricultural Science Kob
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