Method for Estimating the Content of the Chloroform-Extractable Green Fraction (CEGF) in HCl-DMSO Extract of Soils (Soil Chemistry and Mineralogy)

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概要

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• Chloroform-extractable green fraction (CEGF) was detected in the supernatant obtained by alkali precipitation from the HCl-dimethylsulfoxide (DMSO) extract of Pg-rich soil. In the alkaline solution, the color of CEGF was green and CEGF showed strong Pg-like absorption bands. Ultraviolet and visible (UV-VIS) spectral analysis and gel chromatography on Sephadex G-50 were performed to compare several properties between CEGF and Pg. CEGF, which was purified by gel chromatography on Bio-Beads SX-1, displayed strong absorption bands at 609, 562, 445 and 280 nm in the alkaline solution. These absorption bands were almost similar to those of Pg. Furthermore, the UV-VIS spectrum of CEGF in the organic solvents showed a similar characteristic pattern of 4,9-dihydroxyperylene-3,10-quinone (DHPQ), which was considered to be a chromophore of Pg. Based on the results of gel chromatography on Sephadex G-50, CEGF mainly consisted of two fractions, corresponding to the G_2 and G_3 fractions of Pg. These results indicated that the method for extracting CEGF from Pg-rich soil in the present study was easy and selective and that CEGF was one of the components of, or a closely related substance to Pg. A colorimetric method for the estimation of the CEGF content in soils was developed. The calibration curve of CEGF was linear over a wide range of contents from 2.75 to 220 mg L^<-1>. The CEGF content in twelve samples of various soils was examined. CEGF was detected in all the soil sampled (5 orders) including three samples (3 orders) where Pg was not detected, and the content ranged from 0.07 to 1.66 g kg^<-1>(dry soil). Therefore, the method for estimating the CEGF content in soils developed in the present study was found to be suitable for various soil orders and it was assumed that CEGF occurred in various soil orders.

• 社団法人日本土壌肥料学会の論文

著者

• ASAKAWA Daichi

  The Graduate School of Agricultural Science, Kobe University

• YANAGI Yukiko

  The Graduate School of Agricultural Science, Kobe University

• FUJITAKE Nobuhide

  The Graduate School of Agricultural Science, Kobe University

• Fujitake Nobuhide

  Faculty Of Agriculture Kobe University

• 藤嶽 暢英

  神戸大学大学院農学研究科生命機能科学専攻農環境生物学講座

• Asakawa Daichi

  The Graduate School Of Agricultural Science Kobe University

• Yanagi Yukiko

  The Graduate School Of Agricultural Science Kobe University

• Fujitake N

  Laboratory Of Soil Science Faculty Of Agriculture Kobe University

• Fujitake Nobuhide

  Laboratory Of Soil Science Faculty Of Agriculture Kobe University

• Ohta Hiroyuki

  School Of Agriculture Ibaraki University

• Yanagi Yukiko

  Laboratory Of Soil Science Faculty Of Agriculture Kobe University

• SUZUKI Takeshi

  Laboratory of Soil Science, Faculty of Agriculture, Kobe University

• Otsuka H

  Laboratory Of Soil Science Faculty Of Agriculture Kobe University

• Ohta H

  Department Of Biological Sciences Graduate School Of Bioscience And Biotechnology Tokyo Institute Of

• Ohta Hiroyuki

  Dept. Biol. Sci. Tokyo Inst. Technol.

• Ohta H

  Okayama Univ. Dental School Okayama Jpn

• Ohta H

  Ibaraki University College Of Agriculture

• Kobayashi Takayuki

  The Graduate School of Science and Technology, Kobe University

• Watanabe Makiko

  Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology

• Fujitake N

  Graduate School Of Agricultural Sci. Kobe Univ.

• Fujitake Nobuhide

  The Graduate School Of Agricultural Science Kobe University

• Kobayashi Takayuki

  Research Institute For Environmental Management Technology National Institute Of Advanced Industrial

• Fujitake N

  Kobe Univ. Kobe Jpn

• Watanabe M

  Department Of Energy Sciences Tokyo Institute Of Technology

• Watanabe M

  Faculty Of Horticulture Chiba University

• Ohta H

  Ibaraki Univ. Coll. Agriculture Ibaraki Jpn

• Suzuki Takeshi

  Laboratory Of Soil Science Faculty Of Agriculture Kobe University

• Watanabe Makiko

  Interdisciplinary Graduate School Of Science And Engineering Tokyo Institute Of Technology

• Suzuki Takeshi

  Laboratory Of Microbial Biochemistry Institute For Chemical Research Kyoto University

• Fujitake Nobuhide

  Laboratory Of Soil Science Department Of Agro-bioscience Graduate School Of Agricultural Science Kob

• Watanabe Mikiko

  Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology

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