Expression analysis of activin receptor interacting protein (ARIP) in Xenopus

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概要

• 論文の詳細を見る

• Activin receptor interacting protein (ARIP) is a newly isolated protein from mouse using two-hybrid method. As a result of cDNA cloning of Xenopus ARIP (xARIP), 6448 bp of cDNA sequences were obtained, and deduced amino acid sequence was 84% identical to mouse ARIP (ARIP1). RT-PCR analysis showed that maternal xARIP mRNA gradually decreased after invagination, and zygotic expression of xARIP mRNA started at early tail-bud stage. The spatial expressions of xARIP were localized in eyes, brain and especially in glomus at late tail-bud stage. Microinjection of xARIP mRNA showed no effect on embryonic development, but injection of truncated mRNA of xARIP coding only the fifth PDZ domain showed distinct defect in embryos as follows. The invagination was delayed and gastrulation wasn't completed. At late tail-bud stage, heavy defect of pharynx and heart were observed. Pronephric tubes were formed, but pronephros was deformed and expanded. These phenotypes (except malformation in pronephros), might be able to be explained in the point of defect in predicted pharyngeal endoderm, the fore part of organizer region Microinjection of Antisense morpholino oligo of xARIP (moARIP) showed heavier deformed pronephros and resultant edema. These findings suggest that maternal xARIP mRNA may play a role in pharyngeal endoderm formation, and zygotic xARIP mRNA might participate in the patterning of eye and brain and especially have critical role in pronephros formation.

• 日本組織細胞化学会の論文

著者

• 浅島 誠

  東京大学大学院 総合文化研究科 生命環境科学系

• Okabayashi Koji

  Dept. Of Anatomy Kyorin University School Of Medicine

• Okabayashi Koji

  Jst‐icorp

• Kawakami Hayato

  Department of Anatomy, Kyorin University School of Medicine

• Asashima Makoto

  University Of Tokyo

• Tsuchida Kunihiro

  Inst for Enzyme Research, University of Tokushima

• Shoji Hiroki

  Dept. of Endocrinology, Kagawa Medical University

• Sugino Hiromu

  Inst for Enzyme Research, University of Tokushima

• Kawakami Hayato

  Dept. of Anatomy, Kyorin University School of Medicine

• Asashima Makoto

  Dept. of Life Science, University of Tokyo

• Asashima M

  Sopst Project Japan Science And Technology Agency(jst):department Of Life Science (biology) Graduate

• Asashima Makoto

  Department Of Life Sciences (biology) Graduate School Of Arts And Sciences The University Of Tokyo:i

• Asashima Makoto

  Core Research For Evolutional Science And Technology Japan Science And Technology Corporation

• Asashima Makoto

  Sorst Japan Science And Technology Corporation (jst) The University Of Tokyo:department Of Life Scie

• Asashima M

  Dept. Of Life Science University Of Tokyo

• Asashima Makoto

  Department Of Biology The University Of Tokyo

• Tsuchida Kunihiro

  Inst For Enzyme Research University Of Tokushima

• Asashima Makoto

  Dept. Biophys. And Life Sci. Grad. Sch. Arts & Sci. The Univ. Of Tokyo

• Kawakami Hayato

  Second Dep. Of Anatomy Dep. Of Biochemistry Kyorin Univ. School Of Medicine

• Kawakami Hayato

  Dept. Of Anatomy Kyorin University School Of Medicine

• Shoji Hiroki

  Dept. Of Endocrinology Kagawa Medical University

• Asashima M

  Department Of Life Sciences (biology) Graduate School Of Arts And Sciences University Of Tokyo

• Sugino Hiromu

  Inst For Enzyme Research University Of Tokushima

• Kawakami H

  Department Of Anatomy Kyorin University School Of Medicine

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