The Function of O-GlcNAc Transferase and Diabetes
スポンサーリンク
概要
- 論文の詳細を見る
There are many nuclear and cytoplasmic proteins that are modified by a single O-linked N-acetylglucosamine (O-GlcNAc) moieties at serine or threonine residues. This O-GlcNAc modification is a regulatory modification, analogous to phosphorylation, perhaps in part by transiently capping potential phosphorylation sites. O-GlcNAc transferase (OGT: EC2.4.1), an enzyme responsible for O-GlcNAc modification of proteins, has been characterized, and its cDNA has been cloned. O-GlcNAc transferase mRNA is highly expressed in rat and human pancreas. We previously demonstrated that OGT and O-GlcNAc are abundant in almost all pancreatic cells, and are also abundant in the cells of the islets of Langerhans. We speculate that O-GlcNAc modification of proteins by OGT may be involved in the alucose-sensing mechanism in the pancreas. The hexosamine biosynthesis pathway plays an important role in the induction of insulin resistance. In this pathway, glutamine:fructose-6-phosphate amidotransferase (GFAT) plays an integral role in the development of insulin resistance. OGT utilizes cytoplasmic UDP-GlcNAc, which is synthesized in the hexosamine biosynthetic pathway. The increase in hexosamine biosynthesis may stimulate OGT activity, inducing O-GlcNAc modification of proteins that are involved in the glucose transport system or in granule secretion. So it is likely that the effects of hyperglycemia may result from abnormal O-GlcNAc modification. In the present study, we aimed to examine immunohisto-chemichally the expression of O-GlcNAc transferase in the diabetic pancreas by using GK rat as a model animal of the type II diabetes. In the GK rat diabetic pancreas, a loss of beta cells and an increase of collagen fibers were observed. The diabetic pancreas showed an increase in the expression of O-GlcNAc transferase. These findings indicate that the change of O-GlcNAc transferase expression correlates closely with diabetes.
- 日本組織細胞化学会の論文
著者
-
Kawakami Hayato
Department Of 2nd Anatomy Kyorin University School Of Medicine
-
Akimoto Yoshihiro
Department Of Anatomy Kyorin University School Of Medicine
-
Kawakami Hayato
Kyorin Univ. School Of Medicine Tokyo Jpn
関連論文
- Proteomic Analysis of Two Types of Exosomes in Human Whole Saliva
- Co-localization of Human Growth Hormone and Human Prolactin in Hormonally Pure Human Growth Hormone-producing Pituitary Adenomas
- Nephrin and Podocin Expression Around the Onset of Puromycin Aminonucleoside Nephrosis
- Cell-surface arylsulfatase A and B on sinusoidal endothelial cells, hepatocytes, and Kupffer cells in mammalian livers
- MS2-4 Morphometrical evaluation of androgen action in testis and prostate
- Immunohistochemical Localization of β-1, 4-Galactosyltransferase in Human Pancreatic Tissues
- Identification of a single cell-derived bone marrow cell line with transdifferentiation potential for cardiomyocytes
- Exosome-Like Vesicles with Dipeptidyl Peptidase IV in Human Saliva(Biochemistry)
- Myosin Light Chain Phosphorylation is Correlated with Cold-Induced Changes in Platelet Shape
- Growth Inhibition of Helicobacter pylori by Monoclonal Antibody to Heat-Shock Protein 60
- Physiologic Role of Decidual β_1 Integrin and Focal Adhesion Kinase in Embryonic Implantation
- Detection of Disialoganglioside in Rat Cerebellar Cortex by Light and Electron Microscopy
- P3-31 Distribution of the Long Form of Type XII Collagen during Chicken Corneal Formation
- Binding of Griffonia simplicifolia I-B_4 Lectin in the Substantia Gelatinosa of the Rat Spinal Cord
- P-47 Binding of Griffonia simplicifolia I-B_4 lectin in the substantia gelatinosa of rat spinal cord.
- Subcellular Localization of Galactosyltransferase Associated with Tumors in Endometrial and Ovarian Cancer Cells
- Effect of probiotic bacterial strains of Lactobacillus, Bifidobacterium, and Enterococcus on enteroaggregative Escherichia coli
- O-GlcNAc modification of nucleocytoplasmic proteins and diabetes
- Elevated Post-Translational Modification of Proteins by O-Linked N-Acetylglucosamine in Various Tissues of Diabetic Goto-Kakizaki Rats Accompanied by Diabetic Complications
- P-36-A Diabetic keratopathy and O-GlcNAc transferase in corneas of diabetic Goto-Kakizaki rat(THE JOINT MEETING OF THE 44TH ANNUAL MEETING OF THE JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY AND THE 35TH ANNUAL MEETING OF THE CLINICAL ELECTRON MICROS
- Phenotypic Alteration of Carbohydrate Antigens in Gynecological Malignancy
- Lectin-binding Patterns of the Normal and Malignant Endometrium.
- 29-II-07 Effects of steroid on the immunohistochemical expression of activated eosinophil cationic protein(THE JOINT MEETING OF THE 44TH ANNUAL MEETING OF THE JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY AND THE 35TH ANNUAL MEETING OF THE CLINICAL EL
- Importance of Carbohydrates and Platelets in the Early Events of Cancer Metastasis (Recent Progresses in Carbohydrate Histochemistry)
- Changes in Lectin-binding Patterns of the Rat Gastric Mucosa Induced by Stress Ulcer
- Changes in Lectin Binding Patterns of Human Pulmonary Tissues in Association with Congenital Heart Diseases
- B-18 Histochemical Detection of Various Gangliosides in Rat Cerebellar Cortex
- Distribution of glycoconjugates in the colon carcinoma cells during the process of liver metastasis
- DBA-staining Pattern of Stimulated Human Gastric Parietal Cells : A Microspectrophotometric Study
- Expression of Hex homeobox gene during skin development : Increase in epidermal cell proliferation by transfecting the Hex to the dermis
- P-57 Immunohistochemical study on cellular co-localization of estrogen and progesterone receptors, HSP90, and pS2 in breast cancer tissues
- Transdifferentiation of Epidermis to Mucous Epithelium by Retinol Accompanies Increase in Transglutaminase 2/Gh and Decrease in Transglutaminase 3
- Immunohistochemical Localization of β-1, 4-Galactosyltransferase in Human Colonic Tissues
- Distribution of O-GlcNAc Transferase in the Rat Pancreas
- P-48 Glucose-induced Changes in O-GlcNAc Transferase Expression and Localization in Rat Aortic Smooth Muscle Cell
- The Function of O-GlcNAc Transferase and Diabetes
- Lectin Histochemical Study of Squamous Carcinoma in the Head and Neck Region
- P1-36 Change of Long-Spacing Collagen in Descemet's Membrane of Diabetic Goto-Kakizaki Rats and Its Suppression by Antidiabetic Agents
- O-GlcNAc Transferase in Pancreas of Rats : Elevated expression of O-GlcNAc Transferase with Streptozotocin-induced Diabetes
- Changes in Lectin Binding Patterns in Rat Testis and Epididymis in Association with Experimentally-Induced Cryptorchidism
- Okadaic Acid Induces Marked Shape Change of Human Platelets.
- Mechanism of the Inhibitory Action of Steroids Against Activated Eosinophils
- Expression of matrix metalloproteinases 2 and 9 and tissue inhibitors of matrix metalloproteinases 2 and 1 in the glomeruli of human glomerular diseases : the results of studies using immunofluorescence, in situ hybridization, and immunoelectron microscop