Bioassay of Human Granulocyte Colony-Stimulating Factor Using Human Promyelocytic HL-60 Cells
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概要
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A new method for an assay of human granulocyte colony-stimulating factor (hG-CSF) has been developed using human promyelocytic HL-60 cells. The proliferation of HL-60 cells had been suppressed by the addition of dimethyl sulfoxide (DMSO) or retinoic acid (RA). These differentiating agent-treated HL-60 cells exhibited an increase in their number in response to recombinant hG-CSF (rhG-CSF). Neither dibutyl-cAMP nor interferon-γ (IFN-γ)-treated HL-60 cells, however, showed an increase in their number in response to rhG-CSF. The proliferation rate of DMSO-pretreated HL-60 cells was linearly increased from 0.3 to 10 ng/ml of rhG-CSF. L-Value of HL-60 cells assay was 0.027±0.012. The activities of non-glycosylated rhG-CSF produced by Escherichia coli and glycosylated rhG-CSF produced by chinese hamster ovary (CHO) cells were compared using DMSO-treated HL-60 cells; no significant difference between them. DMSO-treated HL-60 cells also responded to interleukin-3 (IL-3) and granulocyte-macrophage colony-stimulating factor (GM-CSF), but did not respond to erythropoietin or macrophage colony-stimulating factor, suggesting that the responsiveness of these cells to growth factor is restricted to myelogenic cytokines. In conclusion, DMSO- or RA-treated HL-60 cells are useful for the measurement of bioactivity of hG-CSF.
- 公益社団法人日本薬学会の論文
- 1997-09-15
著者
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HAYAKAWA Takao
Division of Biological Chemistry and Biologicals, National Institute of Health Sciences
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Yamaguchi Teruhide
Division of Biological Chemistry and Biologicals, National Institute of Health Sciences
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Yamaguchi T
Division Of Cellular And Gene Therapy Products National Institute Of Health Sciences
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Yamaguchi Teruhide
Division Of Biological Chemistry And Biologicals National Institute Of Health Sciences
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Hayakawa T
Pharmaceutical Res. And Technol. Inst. Kinki Univ.
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Hayakawa T
Division Of Biological Chemistry And Biologicals National Institute Of Health Sciences
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Hayakawa T
National Institute Of Health Sciences
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Hayakawa Takao
Division Of Biological Chemistry And Biologicals National Institute Of Health Sciences
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Yamaguchi Teruhide
National Institute Of Health Science
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Kogi Mieko
Division Of Biological Chemistry And Biologicals National Institute Of Health Sciences (formerly Nat
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Yamamoto Y
Faculty Of Agriculture Kochi University
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Yamamoto Yoshinori
Kochi Univ. Kochi Jpn
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YAMAGUCHI Takamasa
Division of Biological Chemistry and Biologicals, National Institute of Health Sciences (formerly Na
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YAMAMOTO Yukio
Division of Cancer Therapeutics, Tokyo Metropolitan Institute of Medical Sciences
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Hazato Tadahiko
Department Of Medical Biology Tokyo Metropolitan Institute Of Medical Science
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Yamaguchi Teruhide
Division of Biological Chemistry & Biologicals, National Institute of Health Sciences
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