Effects of Calyculin A and FK506 on the O_2^- Generation of Rat Peritoneal Macrophages
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概要
- 論文の詳細を見る
To investigate the role of phosphatase in O_2^- generation, the effects of the potent phosphoprotein phosphatase inhibitors, Calyculin A and FK506, were analyzed during phagocytosis using rat peritoneal macrophages. O_2^- generation was continuously measured after addition of opsonized zymosan (op. ZY) or IgG-coated zymosan (IgG-ZY). The rate of O_2^- generation was directly proportional to the number of macrophages, up to1-2×10^6 cells/ml. It was found that the rate and duration of O_2^- generation were markedly inhibited by Calyculin A. The addition of 100nM of Calyculin A reduced O_2^- generation to about one-tenth of the control value. In contrast, FK506 did not inhibit O_2^- generation, suggesting that calcium calmodulin phosphatase is not involved in the activation of NADPH oxidase. This rusult indicates that the process of dephosphorylation might involve activation of NADPH oxidase as a control mechanism in phagocytosis by rat peritoneal macrophages. Furthermore, since Calyculin A is an inhibitor of phosphatase 1 and 2A, it is suggested that dephosphorylation may be evoked by these phosphatases.
- 東海大学の論文
著者
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HAYAKAWA Takao
National Institute of Health Sciences
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Yamaguchi T
Division Of Cellular And Gene Therapy Products National Institute Of Health Sciences
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Hayakawa T
Pharmaceutical Res. And Technol. Inst. Kinki Univ.
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Hayakawa T
Division Of Biological Chemistry And Biologicals National Institute Of Health Sciences
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Hayakawa T
National Institute Of Health Sciences
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Yamaguchi Teruhide
National Institute Of Health Science
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Kogi Mieko
Division Of Biological Chemistry And Biologicals National Institute Of Health Sciences (formerly Nat
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OSHIZAWA Tadashi
National Institute of Health Science
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OSHIZAWA Tadashi
Division of Cellular and Gene Therapy Products National Institute of Health Sciences
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KOGI Mieko
School of Medicine, Tokai University
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Hazato Tadahiko
Department Of Medical Biology Tokyo Metropolitan Institute Of Medical Science
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