Efficient Gene Transfer into Human Trophoblast Cells with Adenovirus Vector Containing Chimeric Type 5 and 35 Fiber Protein(Biopharmacy)
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概要
- 論文の詳細を見る
Recombinant adenovirus (Ad) vectors based on Ad type 5 have been widely used for gene transfer experiments. Conventional Ad type 5 vectors have a narrow range of tropism and are limited by the size of the transgene that can be packaged. To overcome these limitations, we previously developed an Ad vector (Ad5/35 vector) containing a chimeric Ad type 5 and 35 fiber protein. In the current study, we evaluated the ability of the Ad5/35 vector to transfer genes into human trophoblast cell lines (JAR, JEG-3 and BeWo cells), which are used as in vitro models of human placenta. We compared the gene transfer efficiency of Ad5/35 to that of conventional Ad vector. We found that expression of CD46, which are receptors for Ad5/35 vector, are higher than that of coxsackievirus and adenovirus receptor in all 3 trophoblast cell lines, as determined by flow cytometry. Next, we compared the transducing activity of Ad5 vector and Ad5/35 vector that each expressed luciferase as a reporter gene. Ad5/35 vector had greater gene transfer activity than the conventional Ad vector in all 3 trophoblast cell lines (1.82-fold in JAR cells, 5.37-fold in BeWo cells, 6.11-fold in JEG-3 cells). Thus, Ad vector that contains chimeric type 5 and 35 fiber protein can be a powerful tool for gene transfer experiments in human trophoblast cell lines.
- 公益社団法人日本薬学会の論文
- 2004-12-01
著者
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HAYAKAWA Takao
National Institute of Health Sciences
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近藤 昌夫
Graduate School Of Pharmaceutical Sciences Osaka University:department Of Pharmaceutics And Biopharm
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KOIZUMI Naoya
Department of Radiology, Niigata University School of Medicine
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近藤 昌夫
Graduate School Of Pharmaceutical Sciences Osaka Univ.
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Fujii Makiko
Department of Pharmaceutics and Biopharmaceutics, Showa Pharmaceutical University
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Watanabe Yoshiteru
Department of Pharmaceutics and Biopharmaceutics, Showa Pharmaceutical University
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Fujii Makiko
Showa Pharmaceutical Univ.
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Fujii Makiko
Department Of Chemistry Faculty Of Engineering Gifu University
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KONDOH Masuo
Department of Biopharmaceutics and Pharmaceutics, Showa Pharmaceutical University
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Kawase M
Research And Development Center Kobayashi Pharmaceutical Co. Ltd.
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Fujii M
Department Of Pharmaceutics And Biopharmaceutics Showa Pharmaceutical University
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Kawase Masaya
Faculty Of Education Kagawa University
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MIZUGUCHI Hiroyuki
Project III, National Institute of Health Sciences, Osaka Branch, Fundamental Research Laboratories
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Watanabe Yoshiteru
Department Of Pharmaceutics And Biopharmaceutics Showa Pharmaceutical University
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Watanabe Y
Showa Pharmaceutical Univ.
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Koizumi Naoya
Showa Pharmaceutical Univ.
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Fujii M
Showa Univ. Tokyo Jpn
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Mizuguchi Hiroyuki
Lab. Of Gene Transfer And Regulation National Inst. Of Biomedical Innovation
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Mizuguchi Hiroyuki
Project Iii National Institute Of Health Sciences Osaka Branch Fundamental Research Laboratories For
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Koizumi N
Research Department Teikoku Hormone Mfg. Co. Ltd.
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Watanabe Yoshiteru
Showa Pharmaceutical Univ.
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Koizumi Naoya
Department Of Bioengineering Tokyo Institute Of Technology
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Mizuguchi H
Laboratory Of Gene Transfer And Regulation National Institute Of Biomedical Innovation:graduate Scho
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