Polyethyleneimine/Chitosan Hexamer-Mediated Gene Transfection into Intestinal Epithelial Cell Cultured in Serum-Containing Medium(MEDICAL BIOTECHNOLOGY)
スポンサーリンク
概要
- 論文の詳細を見る
In search of an efficient nonviral vector, polyethyleneimine (PEI)-based vectors were examined. In general, the transfection efficiency of nonviral vectors is suppressed by serum. Here we show that PEI based vectors, particularly, the chitosan hexamer-PET vector, could perform efficient gene transfection into intestinal epithelial cells (IEC-6) in the presence of serum. The conjugation order of the two polymers with a plasmid (first, chitosan hexamer; second, PEI) was found to be an important factor in enhancing transfection efficiency.
- 社団法人日本生物工学会の論文
- 2002-07-25
著者
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Kawase Masaya
Graduate School Of Pharmaceutical Sciences Osaka Univ.
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HAYASHI Yuka
Graduate School of Human and Comprehensive Human Sciences, Tsukuba University
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Ouji Yukiteru
大阪大学 薬研究
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Ouji Yukiteru
Graduate School Of Pharmaceutical Sciences Osaka University:(present Address)department Of Parasitol
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Kawase Masaya
Nagahama Institute of Bio-science and Technology
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Yagi Kiyohito
Graduate School of Pharmaceutical Sciences, Osaka University
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Yagi K
Graduate School Of Pharmaceutical Sciences Osaka University
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Yagi Kiyohito
Graduate School Of Pharmaceutical Sciences Osaka Univ.
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Yagi K
Osaka Univ. Osaka Jpn
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Kawase M
Nagahama Institute Of Bio-science And Technology
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Kawase M
Osaka Univ. Osaka Jpn
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Hayashi Yuka
Graduate School Of Pharmaceutical Sciences Osaka University
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MIYAZAKI JUN-ICHI
Division of Stem Cell Regulation Research, G6, Osaka University Medical School
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Yamato E
Osaka University Medical School
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Yamato Eiji
Department Of Geriatric Medicine Osaka University Medical School
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Yamato Eiji
Division Of Stem Cell Regulation Research (g6) Osaka University Graduate School Of Medicine
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Kawase Masaya
Laboratory Of Industrial Biochemistry Department Of Industrial Chemistry Faculty Of Engineering Kyot
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Kawase Masaya
Department Of Bioscience Nagahama Institute Of Bio-science And Technology
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Miyazaki J
Osaka University Medical School
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Yagi Kiyohito
Department Of Biocehmical Engineering Faculty Of Pharmaceutical Sciences Osaka University
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Kawase Mitsuo
Ngk Insulators Ltd.
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Kawase M
Research And Development Center Kobayashi Pharmaceutical Co. Ltd.
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YOSHIDA-TERAKURA AYAKO
Graduate School of Pharmaceutical Sciences, Osaka University
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MAEDA ISAMU
Graduate School of Pharmaceutical Sciences, Osaka University
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Yoshida-terakura Ayako
Graduate School Of Pharmaceutical Sciences Osaka University
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Miyazaki Jun-ichi
Division Of Stem Cell Regulation Research (g6) Osaka University Graduate School Of Medicine
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Maeda Isamu
Laboratory Of Bio-functional Molecular Chemistry Graduate School Of Pharmaceutical Sciences Osaka Un
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Maeda Isamu
Graduate School Of Pharmaceutical Sciences Osaka University
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Hosaka Yoshifumi
Graduate School Of Pharmaceutical Sciences Osaka University
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Maeda Isamu
Graduate School of Agricultural Science, Utsunomiya University
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