In Vivo Cloning of a Pseudomonas oxalaticus σ^<54>-Like Gene Acting in Expression of Hydrogenase Genes From Alcaligenes hydrogenophilus

概要

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In Pseudomonas oxalaticus OX1,which can express the hydrogenase genes from the H_2-oxidizing bacterium Alcaligenes hydrogenophilus, a σ^<54>-like factor-defective mutant was isolated by insertion of the kanamycin resistance marker. The P. oxalaticus OX1 chromosome gene complementing the mutation was cloned in vivo using the R-plasmid R68.45. The recombinant plasmids formed in strain OX1,the R68.45-prime carrying a σ^<54>-like gene, were selected using intrastrain mating of strain OX1. Carrying one of the recombinant plasmids, the σ^<54>-like factor-defective mutant recovered the abilities to express the hydrogenase genes and to assimilate nitrate as the nitrogen source and C_4-dicarboxylates as the carbon sources for growth.
社団法人日本生物工学会の論文
1992-11-25