Increase in Thioredoxin Activity of Intestinal Epihelial Cells Mediated by Oxidative Stress
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概要
- 論文の詳細を見る
Hydrogen peroxide was cytotoxic to the small intenstine epithelial cell line, IEC-6,as judged from an MTT assay and the release of lactate dehydrogenase. The glutathione S-transferase and thioredoxin reductase activities and SH content decreased dose-dependently with H_2O_2,but thioredoxin activity increased at low H_2O_2 concentrations. In addition, the increase in thioredoxin activity was time-dependent during the initial stages of oxidative stress. A reverse transcription-polymerase chain reaction (RT-PCR) amplification also showed that the mRNA content in IEC-6 cells increased time-dependepntly at 0.25 mM H_2O_2. These results indicate that cellular oxidative shock causes an increase in the activity of thioredoxin, which is involved in the defense mechanism against oxidative stress.
- 公益社団法人日本薬学会の論文
- 1999-09-15
著者
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Yagi K
Graduate School Of Pharmaceutical Sciences Osaka University
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Yagi K
Osaka Univ. Osaka Jpn
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Yagi Kiyohito
Department Of Biocehmical Engineering Faculty Of Pharmaceutical Sciences Osaka University
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Yagi Kiyohito
Laboratory Of Bio-functional Engineering Faculty Of Pharmaceutical Sciences Osaka University
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Mizoguchi T
Gakushuin Univ. Tokyo Jpn
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Mizoguchi T
Laboratory Of Bio-functional Molecular Chemistry Graduate School Of Pharmaceutical Sciences Osaka Un
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Mizoguchi Tadashi
Graduate School Of Pharmaceutical Sciences Osaka University
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Mizoguchi Tadashi
Laboratory Of Biochemistry Faculty Of Pharmaceutical Sciences Osaka University
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HIGASHIKUBO Ayako
Laboratory of Bio-Functional Molecular Chemistry, Graduate School of Pharmaceutical Sciences, Osaka
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TANAKA Noriyuki
Laboratory of Bio-Functional Molecular Chemistry, Graduate School of Pharmaceutical Sciences, Osaka
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NODA Naoto
Laboratory of Bio-Functional Molecular Chemistry, Graduate School of Pharmaceutical Sciences, Osaka
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MAEDA Isamu
Laboratory of Bio-Functional Molecular Chemistry, Graduate School of Pharmaceutical Sciences, Osaka
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NANRI Hiroki
Department of Health Development, University of Occupational and Environmental Health
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Maeda Isamu
Laboratory Of Bio-functional Molecular Chemistry Graduate School Of Pharmaceutical Sciences Osaka Un
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Maeda Isamu
Graduate School Of Pharmaceutical Sciences Osaka University
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Noda Naoto
Laboratory Of Bio-functional Molecular Chemistry Graduate School Of Pharmaceutical Sciences Osaka Un
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Nanri Hiroki
Department Of Biochemistry Kyushu University School Of Medicine
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Mizoguchi Tadashi
Laboratory Of Bio-functional Engineering Faculty Of Pharmaceutical Sciences Osaka University
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Nanri H
Department Of Health Development University Of Occupational And Environmental Health
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Mizoguchi Tadaaki
Research Center Of Supercritical Fluid Technology/research Institute For Environmental Conservation
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Higashikubo Ayako
Laboratory Of Bio-functional Molecular Chemistry Graduate School Of Pharmaceutical Sciences Osaka Un
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Tanaka N
Laboratory Of Bio-functional Molecular Chemistry Graduate School Of Pharmaceutical Sciences Osaka Un
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Mizoguchi Tadashi
Faculty Of Pharmaceutical Sciences Osaka University
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Higasikubo Ayako
Laboratory of Bio-Functional Molecular Chemistry, Graduate School of Pharmaceutical Sciences, Osaka University
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