Glucose Transporter Mediation Responsible for Morphological Changes of Human Epithelial Cells on Glucose-Displayed Surfaces(CELL AND TISSUE ENGINEERING)
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概要
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Cellular morphology is one of the important factors for coordinating cell signaling. In this study, the morphological variation via glucose transporter (GLUT)-mediated anchoring was investigated in the cultures of human mammary epithelial cells in the presence or absence of insulin on culture surfaces with the changed ratios of D- and L-glucose displayed. With increasing ratio of D-glucose displayed on the surfaces, the cells showed a stretched shape in the culture with 10 μg/cm^3 insulin, reaching the highest extent of cell stretching at 100% D-glucose display, whereas round cells were predominant at 0% D-glucose display. In the absence of insulin, on the other hand, the extent of cell stretching showed a concave profile in terms of the ratio of D-glucose displayed, the extent being highest at 50% D-glucose display. Blocking of integrin α_5β_1 or GLUTs1 and 4 on the cells using corresponding antibodies revealed that the primary mechanism for cell attachment was based on integrin-mediated binding, and that GLUTs1 and 4 contributed largely to morphological changes of cells. Confocal microscopy further revealed that GLUT4 localization occurred in response to D-glucose display as well as insulin addition. In the absence of insulin, GLUT4 spots were extensively observed in the cell body regardless of whether D-glucose was displayed or not. However, in the presence of insulin, the broad distribution of GLUT4 appeared on the basal and apical sides of cells at 100% D-glucose display, in contrast with its localization only on the apical side of cells at 0% D-glucose display. These results suggest that the quantitative balance between GLUTs on the cytoplasmic membrane and D-glucose displayed on a culture surface determines the cell morphology, as explained by the receptor saturation model.
- 社団法人日本生物工学会の論文
- 2008-04-25
著者
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紀ノ岡 正博
阪大院・工・生命先端
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近藤 昌夫
Graduate School Of Pharmaceutical Sciences Osaka University:department Of Pharmaceutics And Biopharm
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Kawase Masaya
Graduate School Of Pharmaceutical Sciences Osaka Univ.
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田谷 正仁
大阪大 大学院基礎工学研究科
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田谷 正仁
大阪大学大学院基礎工学研究科 化学系専攻
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Kino-oka Masahiro
Division of Chemical Engineering, Graduate School of Engineering Science, Osaka University
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紀ノ岡 正博
Dep. Of Biotechnology Graduate School Of Engineering Osaka Univ. 2-1 Yamadaoka Suita Osaka 565-0871
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Kino-oka Masahiro
Grad. Sch. Eng. Sci. Osaka Univ.
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近藤 昌夫
Graduate School Of Pharmaceutical Sciences Osaka Univ.
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Kawase Masaya
Nagahama Institute of Bio-science and Technology
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Yagi Kiyohito
Graduate School of Pharmaceutical Sciences, Osaka University
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Yagi K
Graduate School Of Pharmaceutical Sciences Osaka University
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Yagi Kiyohito
Graduate School Of Pharmaceutical Sciences Osaka Univ.
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Yagi K
Osaka Univ. Osaka Jpn
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Kawase M
Nagahama Institute Of Bio-science And Technology
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Kawase M
Osaka Univ. Osaka Jpn
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Taya Masahito
Div. Of Chemical Engineering Graduate School Of Engineering Sci. Osaka Univ. 1-3 Machikaneyama-cho T
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Taya M
Nagoya Univ. Nagoya Jpn
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Taya Masahito
Division Of Chemical Engineering Graduate School Of Engineering Science Osaka University
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Taya Masahito
Department Of Chemical Engineering Faculty Engineering Science Osaka University
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Kim Mee-Hae
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Kawase Masaya
Faculty of Pharmaceutical Sciences, Osaka-ohtani University
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Taya Masahito
Department Of Chemical Science And Engineering Graduate School Of Engineering Science
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Taya Masahito
Division Of Chemical Engineering Department Of Materials Science Engineering Graduate School Of Engi
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Taya Masahito
Department Of Chemical Engineering Faculty Of Engineerig Nagoya University
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Kim Mee-hae
Division Of Chemical Engineering Graduate School Of Engineering Science Osaka University
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Kim Mee-hae
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Kawase Masaya
Laboratory Of Industrial Biochemistry Department Of Industrial Chemistry Faculty Of Engineering Kyot
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Yagi Kiyohito
Department Of Biocehmical Engineering Faculty Of Pharmaceutical Sciences Osaka University
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Kino-oka Masahiro
Division Of Chemical Engineering Department Of Biotechnology Graduate School Of Engineering Osaka Un
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Kawase Mitsuo
Ngk Insulators Ltd.
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Kawase M
Research And Development Center Kobayashi Pharmaceutical Co. Ltd.
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Kawase Masaya
Faculty Of Education Kagawa University
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Taya Masahito
Department Of Biotechnology Graduate School Of Engineering Osaka University
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田谷 正仁
阪大院・基礎工:英文誌編集委員会:電子情報編集委員会
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Kawase Masaya
Faculty Of Bioscience Nagahama Institute Of Bio-science And Technology
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