Growth and differentiation potentials in confluent state of culture of human skeletal muscle myoblasts(CELL AND TISSUE ENGINEERING)

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The transitional behaviors of myoblasts toward differentiation were investigated in the cultures at the low and high seeding densities (respectively, X_0=1.0×10^3 and 2.0×10^5cells/cm^2). In the culture at the low seeding density, an increase in confluence degree accompanied a decrease in growth potential (R_p), being R_p=0.85 and 0.11 at t=48 and 672h, respectively. Myoblasts seeded at the high density resulted in the immediate cessation of growth with keeping the low range of R_p=0.02-0.09 throughout the culture. The reduction of R_p led to the generation of three subpopulations of cells in proliferative, quiescent and differentiated states. Close cell contacts in the confluent state of high seeding culture induced cell quiescence to a higher extent with suppressing differentiation.