Coexpression of Platelet-Derived Growth Factor Receptor Alpha and Fetal Liver Kinase 1 Enhances Cardiogenic Potential in Embryonic Stem Cell Differentiation In Vitro(CELL AND TISSUE ENGINEERING)

概要

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Nascent mesodermal cells derived from EB5 embryonic stem (ES) cells were sorted in terms of cardiogenic potential on the basis of their expression levels of platelet-derived growth factor receptor alpha (PDGFRα) and fetal liver kinase 1 (Flk-1). The sorted cells were cocultured with OP9 stromal cells to induce terminal differentiation into contractile cardiac colonies. A significant number of cardiac colonies were found in the Flk-1+/PDGFRα+ fraction. The enrichment double-positive fraction produced approximately five fold more cardiac colonies than the Flk-1+/PDGFRα-fraction and 10-fold more than the Flk-1-/PDGFRα+ fraction. To investigate the involvement of these markers in embryonic cardiogenesis, the cells that disseminated from the E7.5-7.75 embryos were fractionated and seeded on OP9 cells. The cardiogenic potential was markedly enhanced in the Flk-1+/PDGFRα+ fraction. These results suggest that some of the precursor cells coexpressing these markers are selectively involved in cardiogenic events, and that the identification of ES-cell-derived precursors with these markers will contribute to the effective production of cardiomyocytes for cell therapies.
社団法人日本生物工学会の論文
2007-05-25